Survival of Burkholderia pseudomallei in water.

BMC Research Notes
Richard A MooreDonald E Woods

Abstract

The ability of Burkholderia pseudomallei to survive in water likely contributes to its environmental persistence in endemic regions. To determine the physiological adaptations which allow B. pseudomallei to survive in aqueous environments, we performed microarray analyses of B. pseudomallei cultures transferred from Luria broth (LB) to distilled water. Increased expression of a gene encoding for a putative membrane protein (BPSL0721) was confirmed using a lux-based transcriptional reporter system, and maximal expression was noted at approximately 6 hrs after shifting cells from LB to water. A BPSL0721 deficient mutant of B. pseudomallei was able to survive in water for at least 90 days indicating that although involved, BPSL0721 was not essential for survival. BPSL2961, a gene encoding a putative phosphatidylglycerol phosphatase (PGP), was also induced when cells were shifted to water. This gene is likely involved in cell membrane biosynthesis. We were unable to construct a PGP mutant suggesting that the gene is not only involved in survival in water but is essential for cell viability. We also examined mutants of polyhydroxybutyrate synthase (phbC), lipopolysaccharide (LPS) oligosaccharide and capsule synthesis, and these muta...Continue Reading

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Citations

Apr 24, 2014·Journal of Industrial Microbiology & Biotechnology·Youngbeom AhnCarl E Cerniglia
Jun 26, 2012·Letters in Applied Microbiology·S E Gilbert, L J Rose
Apr 9, 2009·PLoS Neglected Tropical Diseases·Patcharaporn TippayawatGanjana Lertmemongkolchai
Jan 7, 2017·Journal of the American Veterinary Medical Association·Katie PortacciRobert Dobos
Sep 21, 2018·PloS One·Shawn LewenzaShauna Reckseidler-Zenteno
Nov 21, 2019·Chembiochem : a European Journal of Chemical Biology·Lachlan DowBernard Lepetit
Mar 19, 2014·Plant, Cell & Environment·Vladimir SukhovVladimir Vodeneev
May 7, 2013·Annals of Botany·Jan NovákBřetislav Brzobohatý
Mar 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Nicolás E BlancoÅsa Strand
Apr 17, 2020·Clinical Microbiology Reviews·Mariana TavaresIsabel Sá-Correia
Oct 3, 2018·Tropical Medicine and Infectious Disease·Siew Hoon SimGladys Gek Yen Tan
Feb 9, 2021·Frontiers in Microbiology·Chalita ChomkatekaewClaire Chewapreecha

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Methods Mentioned

BETA
PCR
transmission electron microscopy
electron microscopy

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