Survival of Deinococcus geothermalis in Biofilms under Desiccation and Simulated Space and Martian Conditions

Astrobiology
Jan FröslerPetra Rettberg

Abstract

Biofilm formation represents a successful survival strategy for bacteria. In biofilms, cells are embedded in a matrix of extracellular polymeric substances (EPS). As they are often more stress-tolerant than single cells, biofilm cells might survive the conditions present in space and on Mars. To investigate this topic, the bacterium Deinococcus geothermalis was chosen as a model organism due to its tolerance toward desiccation and radiation. Biofilms cultivated on membranes and, for comparison, planktonically grown cells deposited on membranes were air-dried and exposed to individual stressors that included prolonged desiccation, extreme temperatures, vacuum, simulated martian atmosphere, and UV irradiation, and they were exposed to combinations of stressors that simulate space (desiccation + vacuum + UV) or martian (desiccation + Mars atmosphere + UV) conditions. The effect of sulfatic Mars regolith simulant on cell viability during stress was investigated separately. The EPS produced by the biofilm cells contained mainly polysaccharides and proteins. To detect viable but nonculturable (VBNC) cells, cultivation-independent viability indicators (membrane integrity, ATP, 16S rRNA) were determined in addition to colony counts. De...Continue Reading

References

Feb 1, 1992·Pain·R F Catchlove, R A Ramsay
Aug 15, 1991·Analytical Biochemistry·T M Filisetti-Cozzi, N C Carpita
Feb 15, 1987·The Biochemical Journal·J H CroweC Aurell Wistrom
Jan 1, 1985·Applied and Environmental Microbiology·D J Reasoner, E E Geldreich
Feb 1, 1981·Archives of Biochemistry and Biophysics·K Brawn, I Fridovich
Jan 1, 1997·Annual Review of Microbiology·J R Battista
May 1, 1999·Applied and Environmental Microbiology·M O Elasri, R V Miller
May 2, 2000·Applied and Environmental Microbiology·C SignorettoP Canepari
Jun 13, 2000·Journal of Bacteriology·I D AuerbachP A Holden
Feb 13, 2001·Microbiology·I Sutherland
Apr 21, 1988·Nature·H J Melosh
Jan 1, 1992·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·T Ito
Sep 7, 2001·Icarus·C MileikowskyJ Q Zheng
Jan 5, 2002·Journal of Industrial Microbiology & Biotechnology·M KolariM S Salkinoja-Salonen
Mar 8, 2002·Research in Microbiology·Daniela Billi, Malcolm Potts
Apr 12, 2002·Journal of Bacteriology·M KolariM S Salkinoja-Salonen
Jan 7, 2003·Applied and Environmental Microbiology·Hiroyuki KimuraTakeshi Naganuma
Jan 18, 2003·Journal of Microbiological Methods·Kasthuri VenkateswaranRoger Kern
May 6, 2003·The Journal of Biological Chemistry·Diane E HeckJeffrey D Laskin
Dec 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·J-M Monier, S E Lindow
May 12, 2004·International Journal of Food Microbiology·Yi-Chieh WangCheng-Chun Chou
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Apr 1, 1992·Applied and Environmental Microbiology·E B Roberson, M K Firestone
Feb 1, 1994·Applied and Environmental Microbiology·T Ophir, D L Gutnick
Dec 20, 2005·Journal of Industrial Microbiology & Biotechnology·Mari RaulioMirja Salkinoja-Salonen
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Jason M HansenDean P Jones
Apr 4, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M B FrançaE C A Eleutherio
Apr 25, 2006·Journal of Microbiological Methods·C A MorganG Vesey
Sep 19, 2006·Journal of Bacteriology·C SaarimaaP Neubauer
Mar 22, 2007·PLoS Biology·Michael J DalyJames K Fredrickson

❮ Previous
Next ❯

Citations

Jul 9, 2020·Microorganisms·Kyle S LandrySandip S Panesar
Nov 30, 2018·Astrobiology·Olga PodolichJean-Pierre Paul de Vera
Jul 25, 2019·International Journal of Molecular Sciences·Wen YinJin He
Jul 3, 2020·Microbial Biotechnology·Kristie TannerManuel Porcar
Sep 29, 2020·Frontiers in Microbiology·Yuko KawaguchiAkihiko Yamagishi
Mar 30, 2020·Applied and Environmental Microbiology·M PannekensR U Meckenstock
Jun 6, 2020·Frontiers in Microbiology·Tetyana Milojevic, Wolfram Weckwerth
May 1, 2019·Frontiers in Microbiology·Nancy MerinoDonato Giovannelli
Feb 21, 2021·Scientific Reports·Erika BoulantJean Michel Bolla

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
scanning electron microscopy

Software Mentioned

Excel

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

© 2022 Meta ULC. All rights reserved