Survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community

FEMS Microbiology Ecology
Monika GötzKornelia Smalla

Abstract

The survival and colonization patterns of Pseudomonas putida PRD16 and Enterobacter cowanii PRF116 in the rhizosphere of greenhouse-grown tomato plants and the effects of their inoculation on the indigenous bacterial community were followed by selective plating, molecular fingerprinting, and confocal laser scanning microscopy (CLSM) over 3 weeks. Both strains, which showed in vitro antagonistic activity against Ralstonia solanacearum, were previously tagged with gfp. Seed and root inoculation were compared. Although plate counts decreased for both gfp-tagged antagonists, PRD16 showed a better survival in the rhizosphere of tomato roots independent of the inoculation method. Analysis of 16S rRNA gene fragments amplified from total community DNA by denaturing gradient gel electrophoresis and CLSM confirmed the decrease in the relative abundance of the inoculant strains. Pronounced differences in the Pseudomonas community patterns for plants inoculated with PRD16 compared to the control were detected 3 weeks after root inoculation, indicating a longer-lasting effect. Analysis by CLSM showed rather heterogeneous colonization patterns for both inoculant strains. In comparison with seed inoculation, root inoculation led to a much bet...Continue Reading

References

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Nov 1, 2002·FEMS Microbiology Ecology·Janus A J HaagensenS Molin

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Citations

May 25, 2010·Microbial Ecology·Vanessa OliveiraAngela Cunha
Apr 12, 2011·Applied and Environmental Microbiology·Lilach Iasur-KruhDror Minz
Apr 24, 2009·Microbial Ecology·R TrifonovaJ D van Elsas
Aug 18, 2009·International Journal of Systematic and Evolutionary Microbiology·Munusamy MadhaiyanPalani Santhanakrishnan
Jun 10, 2008·FEMS Microbiology Ecology·Newton C Marcial GomesKornelia Smalla
Jul 14, 2010·FEMS Microbiology Ecology·Christin ZachowGabriele Berg
Aug 9, 2016·Waste Management·Louise Hénault-EthierYves Gélinas
Mar 23, 2017·Frontiers in Microbiology·Raquel S PeixotoDavid G Bourne
Dec 7, 2018·The ISME Journal·Phillipe M RosadoRaquel S Peixoto
Dec 18, 2019·International Journal of Molecular Sciences·Da-Cheng WangJian-Hua Guo

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