PMID: 6989777Mar 1, 1980Paper

Survival of irradiated glia and glioma cells studied with a new cloning technique

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
S NilssonJ Ponten

Abstract

A method allowing cloning of monolayer cultured cells with a low plating efficiency was developed. Cells were grown in several small palladium squares to obtain a high cell density. These squares were surrounded by non-adhesive agarose to prevent large distance migration and thereby mixing of the clones. By using easily-cloned hamster cells for comparison it was found that the survival curves were similar to the curves obtained with conventional cloning. The new method was used to compare the radiosensitivity of cultured human glia and glioma cells which both have a low plating efficiency (less than 5 per cent) when seeded sparsely. The survival curves for the glioma cells had high Do-values (1.5--2.5 Gy) and large shoulders (extrapolation numbers around 5) indicating that they were rather resistant and had a high capacity for accumulation of sublethal damage. The survival curves for glia cells had lower Do-values (1.3--1.5 Gy) and no shoulders at all, indicating that they were more sensitive than the glioma cells.

References

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Citations

Jan 1, 1982·Acta Radiologica. Oncology·T Nederman, G Benediktsson
Jun 27, 1998·Acta Oncologica·N E Crompton
Feb 22, 2008·Electrophoresis·Sara LindströmHelene Andersson Svahn
Dec 1, 1990·Journal of Neuro-oncology·J CapalaJ Carlsson
Jul 1, 1991·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·G P RaaphorstL H Gerig
Jan 1, 1983·Acta Radiologica. Oncology·L JohanssonK Nilsson
May 24, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Jörgen CarlssonHans Lundqvist

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