Survival of Listeria monocytogenes strain H7762 and resistance to simulated gastric fluid following exposure to frankfurter exudate

Journal of Food Protection
Laura D Wonderling, D O Bayles

Abstract

Listeria monocytogenes strain H7762, a frankfurter isolate, was tested to determine whether it was able to survive at 4 degrees C in frankfurter pack fluid (exudate) and to determine whether food exposure affects its acid sensitivity. Cultures were sampled and tested for acid sensitivity by challenge with simulated gastric fluid (SGF). SGF challenges performed immediately after inoculation revealed that between 20 and 26% of the cells survived the full 30 min of SGF challenge regardless of whether the cells were inoculated into brain heart infusion broth (BHI) or exudate. After 2 days of incubation, cells exposed to both exudate and BHI had significantly decreased SGF resistance; however, the cells exposed to exudate were significantly more SGF resistant than cells exposed to BHI (after 15 min of SGF treatment, 33% of the exudate-exposed cells survived and 12% of the BHI-exposed cells survived). L. monocytogenes exposed to exudate had greater SGF resistance at all challenge times compared with BHI-exposed cells from day 2 through day 4. From days 8 to 15, exudate-exposed cells continued to have greater SGF resistance than BHI-exposed cells up to 10 min of SGF challenge but were as sensitive as the BHI-exposed cells at 20 to 30 ...Continue Reading

References

Jan 1, 1992·International Journal of Food Microbiology·R R BeumerF M Rombouts
Feb 1, 1991·Molecular Microbiology·K JohnsonC Hormaeche
Jul 1, 1986·Applied and Environmental Microbiology·D E ConnerL R Beuchat
Jun 1, 1994·International Journal of Food Microbiology·B M MackeyJ Baranyi
Aug 15, 1998·Critical Reviews in Food Science and Nutrition·M Peleg, M B Cole
Aug 26, 1998·International Journal of Food Microbiology·M Cheroutre-VialetteA Lebert
Feb 25, 1999·Letters in Applied Microbiology·G A Dykes, K M Withers
Oct 8, 1999·Emerging Infectious Diseases·P S MeadR V Tauxe
Mar 4, 2000·Journal of Food Protection·M Peleg, M B Cole
Mar 23, 2000·Letters in Applied Microbiology·D O Bayles, B J Wilkinson
Apr 19, 2000·Critical Reviews in Food Science and Nutrition·M Peleg, C M Penchina
Jun 17, 2000·International Journal of Food Microbiology·G A Dykes, S M Moorhead

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