Survival of mouse embryos after vitrification depending on the cooling rate of the cryoprotectant solution

Acta Veterinaria Hungarica
R HredzákJ Kacmárik

Abstract

The aim of the study was to determine the relationship between the rate of cooling of eight-cell mouse embryos to the temperature of liquid nitrogen (-196 degrees C) and their developmental capacity after thawing on the basis of their ability to leave the zona pellucida ('hatching') during in vitro culturing. Eight-cell embryos were obtained from superovulated female mice and divided into three experimental and one control group. Embryos from the experimental groups were cryopreserved by the vitrification method using ethylene glycol as cryoprotectant. The vitrification protocols used in the study differed in the rate of cooling of the cryoprotectant solution. Embryos from the first group were frozen in conventional 0.25-ml plastic straws, those from the second group in pipetting 'tips', and embryos from the third group, placed in vitrification solution, were introduced dropwise directly into liquid nitrogen. The control group of embryos was cultured in vitro without freezing in a culturing medium in an environment consisting of 95% air and 5% CO2. The developmental capacity of thawed embryos was assessed on the basis of their ability to leave the zona pellucida ('hatching') after three days of in vitro culturing. In the contro...Continue Reading

References

Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Aug 31, 2001·Human Reproduction·R R YeomanD P Wolf
Aug 9, 2002·Journal of Assisted Reproduction and Genetics·Michael L ReedJim Thompson
Oct 4, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·J Liebermann, M J Tucker
Jan 30, 2004·Reproductive Biomedicine Online·Juergen LiebermannMichael J Tucker
Mar 24, 2005·Acta Veterinaria Hungarica·B BaranyaiElen Gócza

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Citations

Nov 27, 2008·Zygote : the Biology of Gametes and Early Embryos·M PopelkováP Chrenek
Jul 15, 2009·BioTechniques·Wai Hung Tsang, King L Chow
Dec 10, 2019·Clinical and Experimental Reproductive Medicine·Mahla Honari ChatroudiSomayyeh Safari

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