Survival of total coliforms in lawn irrigated with secondary wastewater and chlorinated effluent in the Mediterranean region

Water Environment Research : a Research Publication of the Water Environment Federation
T ManiosD Mantzavinos

Abstract

Pregrown, two-month-old lawn was layered in 12 large square pots with an area of 0.25 m2 each, filled with a mixture of topsoil, peat, and sand. In late July, in the heart of the Mediterranean summer, the pots were divided into two groups, with six pots per group. On four different occasions, the pots in each group were sprinkled (surface irrigation) with 2 L of either secondary-treated wastewater (STW, group A) or chlorinated effluent (CHE, group B). Wastewater application always took place at 0700 hours. Samples of the surface soil and grass from each pot were collected at the following times: before irrigation, immediately after irrigation, two hours later (0900 hours), and four hours later (1100 hours). In the samples collected, the number of total coliforms per gram was measured using standard microbiological analyses. Temperature and sunlight intensity were also monitored. There was an increase in the coliforms population in soil and grass samples of both groups immediately after the wastewater application. In group A, the mean number of coliforms recorded in the soil samples reached mean values higher than 5000 cfu/g compared to 312 cfu/g recorded before application. The increase in group B was smaller but still signific...Continue Reading

References

Jul 27, 1999·Journal of Food Protection·K T Rajkowski, E W Rice
Nov 1, 2000·Chemosphere·J A Herrera MeliánJ Pérez Peña
May 18, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·U J BlumenthalG Ruiz-Palacios
Mar 2, 2002·Applied and Environmental Microbiology·Lester W SintonRobert J Davies-Colley

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