PMID: 9182668Jun 16, 1997Paper

Survival of Trypanosoma brucei in the tsetse fly is enhanced by the expression of specific forms of procyclin

The Journal of Cell Biology
S RueppIsabel Roditi

Abstract

African trypanosomes are not passively transmitted, but they undergo several rounds of differentiation and proliferation within their intermediate host, the tsetse fly. At each stage, the survival and successful replication of the parasites improve their chances of continuing the life cycle, but little is known about specific molecules that contribute to these processes. Procyclins are the major surface glycoproteins of the insect forms of Trypanosoma brucei. Six genes encode proteins with extensive glutamic acid-proline dipeptide repeats (EP in the single-letter amino acid code), and two genes encode proteins with an internal pentapeptide repeat (GPEET). To study the function of procyclins, we have generated mutants that have no EP genes and only one copy of GPEET. This last gene could not be replaced by EP procyclins, and could only be deleted once a second GPEET copy was introduced into another locus. The EP knockouts are morphologically indistinguishable from the parental strain, but their ability to establish a heavy infection in the insect midgut is severely compromised; this phenotype can be reversed by the reintroduction of a single, highly expressed EP gene. These results suggest that the two types of procyclin have di...Continue Reading

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Citations

May 20, 2011·Protoplasma·Angela SchwedeMark Carrington
Dec 21, 2010·Amino Acids·Ehud InbarClaudio A Pereira
Jan 30, 2004·Molecular and Biochemical Parasitology·Karin AsbeckWendy Gibson
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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.