Survival, Retention, and Selective Proliferation of Lymphocytes Is Mediated by Gingival Fibroblasts

Frontiers in Immunology
Carolyn G J MoonenTeun J de Vries

Abstract

Periodontitis, a chronic inflammatory disease of the periodontium, is characterized by osteoclast-mediated alveolar bone destruction. Gingival fibroblasts (GFs) present in the bone-lining mucosa have the capacity to activate the formation of osteoclasts, but little is known about which local immune cells (co-)mediate this process. The aim of this study was to investigate the cellular interactions of GFs with immune cells, including the contribution of GFs to osteoclast formation and their possible role in the proliferation of these immune cells. In addition, we investigated the expression of adhesion molecules and the inflammatory cytokines that are evoked by this interaction. GFs were cocultured with peripheral blood mononuclear cells (PBMCs), CD14+ monocytes or peripheral blood lymphocytes (PBLs) for 7, 14, and 21 days. After 21 days, comparable numbers of multinucleated cells (osteoclasts) were found in gingival fibroblast (GF)-PBMC and GF-monocyte cocultures. No osteoclasts were formed in GF-PBL cocultures, indicating that the PBLs present in GF-PBMC cocultures do not contribute to osteoclastogenesis. Persisting mononuclear cells were interacting with osteoclasts in GF-PBMC cocultures. Remarkably, a predominance of CD3+ T c...Continue Reading

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Citations

Oct 23, 2019·Journal of Immunology Research·Carolyn G J MoonenBruno G Loos
Dec 11, 2019·International Journal of Molecular Sciences·Carolyn G J MoonenTeun J de Vries
Jul 6, 2019·Frontiers in Immunology·Maria-Bernadette MadelClaudine Blin-Wakkach
May 1, 2019·International Journal of Molecular Sciences·Erica CostantiniSergio Caputi
Dec 30, 2020·International Journal of Molecular Sciences·Andreas SeidelHelga Schmetzer

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
fluorescence microscopy
FACS
PCR
enzyme-linked immunosorbent assays
FCS

Software Mentioned

FACSuite
GraphPad Prism
[UNK]

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