Susceptibility of human villous (BeWo) and extravillous (HTR-8/SVneo) trophoblast cells to Toxoplasma gondii infection is modulated by intracellular iron availability

Parasitology Research
Marcos Paulo Oliveira AlmeidaNeide Maria Silva

Abstract

Congenital toxoplasmosis is a serious health problem that can lead to miscarriage. HTR-8/SVneo is a first trimester extravillous trophoblast, while BeWo is a choriocarcinoma with properties of villous trophoblast cells. In the placenta, iron is taken up from Fe-transferrin through the transferrin receptor being the ion an important nutrient during pregnancy and also for Toxoplasma gondii proliferation. The aim of this study was to evaluate the role of iron in T. gondii proliferation in BeWo and HTR-8/SVneo cells and in human chorionic villous explants. The cells were infected with T. gondii, iron supplemented or deprived by holo-transferrin or deferoxamine, respectively, and parasite proliferation and genes related to iron balance were analyzed. It was verified that the addition of holo-transferrin increased, and DFO decreased the parasite multiplication in both trophoblastic cells, however, in a more expressive manner in HTR-8/SVneo, indicating that the parasite depends on iron storage in trophoblastic cells for its growth. Also, tachyzoites pretread with DFO proliferate normally in trophoblastic cells demonstrating that DFO itself does not interfere with parasite proliferation. Additionally, T. gondii infection induced enhanc...Continue Reading

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Citations

Feb 9, 2021·Frontiers in Microbiology·Idessania Nazareth CostaEloisa Amália Vieira Ferro
Feb 9, 2021·Frontiers in Veterinary Science·Iván Pastor-FernándezPilar Horcajo
Jun 30, 2021·Parasitology Research·Seyed Hossein AbdollahiSoudeh Khanamani Falahati-Pour
Aug 28, 2021·Micromachines·Marta CherubiniKristina Haase

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

GraphPad
GraphPad Prism

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