Susceptibility of lipopolysaccharide-responsive and -hyporesponsive ItyS Mice to infection with rough mutants of Salmonella typhimurium.

Infection and Immunity
I Mattsby-BaltzerP de Man

Abstract

The R5 (chemotype Rb) but not the R10 (chemotype Rd) mutant of murine pathogen Salmonella typhimurium 395MS was extremely virulent in intraperitoneal infections of C57BL/10ScCr mice carrying the ityS and lpsD alleles. C57BL/6J (ityS lpsN) and C3H/HeJ (ityR lpsD) mice showed a much higher resistance to the R5 mutant. Further studies were performed with peritoneal macrophages in vitro in order to elucidate susceptibility in lipopolysaccharide (LPS)-hyporesponsive mice carrying ItyS. The intracellular killing capacity of the ItyS LpsD macrophages was lower than that of the ItyS LpsN macrophages for the R5 mutant and may partly explain the increased susceptibility of the ItyS LpsD mice. The deep rough mutant, R10, was rapidly killed intracellularly by the ItyS LpsD macrophages. Processing of the bacteria in macrophages that had phagocytosed R5 or R10 bacteria was followed for up to 18 days by endotoxin measurements (limulus assay) and immunostaining, with monoclonal antibodies to various parts of the LPS molecule being used. Only 0.1% or less of the macrophage-associated bacteria remained alive after 48 h of incubation, and none were alive on day 7. Although immunostaining showed that LPS was present in both the LpsD and LpsN macro...Continue Reading

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Citations

Feb 6, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ya-Ching HsiehIrshad H Chaudry
Nov 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S LangeI Mattsby-Baltzer
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrés Vazquez-TorresFerric C Fang

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