Abstract
Doxycycline is used in combination with quinine for malaria treatment or alone for malaria chemoprophylaxis. However, the occurrence of malaria after doxycycline chemoprophylaxis has been reported. Identification of genetic determinants that contribute to the susceptibility of Plasmodium falciparum to doxycycline will be important for the detection and surveillance of doxycycline resistance. Sequence analysis of 11 genes (pftufA, pfEF-TS, pfmdt, pftetQ, pfrps3, pfrps7, pfrps8, pfrps9, pfrps11, pfrps14, and pfrps17) and evaluation of pfmdt and pftetQ copy numbers by quantitative real-time polymerase chain reaction were conducted in 90 African P. falciparum isolates that were obtained from 14 countries and that belonged to phenotypic groups differing in their doxycycline median inhibitory concentrations. We found that pfmdt copy number of >1 (adjusted odds ratio [OR], 7.09 [95% confidence interval {CI}, 1.58-31.82]; P=.011), pftetQ copy number of >1 (adjusted OR, 5.23 [95% CI, 1.06-25.77]; P=.042), and KYNNNN amino acid motif repeats of <3 (adjusted OR, 3.00 [95% CI, 1.02-8.86]; P=.046) were independently associated with decreased susceptibility to doxycycline. Our findings suggest that pfmdt and pftetQ copy numbers and pftetQ se...Continue Reading
Citations
Oct 27, 2011·Chemical Reviews·Cyrille Y BottéChristophe Biot
Jun 16, 2010·Antimicrobial Agents and Chemotherapy·Sébastien BriolantBruno Pradines
May 18, 2011·Antimicrobial Agents and Chemotherapy·Richard T EastmanDavid A Fidock
May 4, 2012·Malaria Journal·Nathalie WurtzSébastien Briolant
Nov 15, 2013·Malaria Journal·Tiphaine GaillardUNKNOWN French National Reference Centre for Imported Malaria Study Group
May 27, 2010·Malaria Journal·Sébastien BriolantBruno Pradines
Apr 5, 2011·The American Journal of Tropical Medicine and Hygiene·Kathrine R TanUNKNOWN Centers for Disease Control and Prevention
May 28, 2013·International Journal of Cell Biology·Jasmin LindnerCarsten Wrenger
Jul 30, 2014·Travel Medicine and Infectious Disease·R MiglianiC Rapp
Aug 1, 2013·Molecular Microbiology·Philip J Rosenthal
Sep 18, 2015·Malaria Journal·Tiphaine GaillardUNKNOWN French National Reference Centre for Imported Malaria Study Group
Jul 25, 2015·Malaria Journal·Marie MuraEric Legrand
Jul 22, 2015·Emerging Infectious Diseases·Marylin MadametBruno Pradines
Apr 27, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T GaillardB Pradines
Sep 21, 2016·Malaria Journal·Jean Baptiste RoseauEmilie Javelle
Oct 30, 2016·Trends in Parasitology·Christopher D GoodmanStuart A Ralph
Feb 10, 2016·Antimicrobial Agents and Chemotherapy·Emilie JavelleBruno Pradines
Aug 4, 2018·Canadian Journal of Physiology and Pharmacology·Vinoth RajendranPrahlad C Ghosh
Nov 12, 2015·Malaria Journal·Tiphaine GaillardBruno Pradines
Nov 9, 2019·Biological Reviews of the Cambridge Philosophical Society·Rowena E Martin
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diana FontinhaMiguel Prudêncio
May 15, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sahil KumarRajesh K Singh