Susceptibility of sodA- and sodB-deficient Escherichia coli mutant towards antimicrobial photodynamic inactivation via the type I-mechanism of action

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Pouriya Faraj TabriziTim Maisch

Abstract

Photodynamic antimicrobial chemotherapy (PACT) is a multi-target method to inactivate pathogenic microorganisms by exciting a photosensitizer (PS) with visible light of appropriate wavelength in the presence of molecular oxygen (3O2). There are two major pathways by which reactive oxygen species (ROS) are produced. In type I (TI)-reactions, radicals such as superoxide (O2˙-) and hydroxyl radicals (˙OH) are generated by electron transfer. In type II (TII)-reactions, highly reactive singlet oxygen (1O2) is produced by direct energy transfer. This study investigated the efficiency of PACT in Gram-negative Escherichia coli wild type (EC WT) and the mutant Escherichia coli PN134 (EC PN134) which is not able to produce SOD A and SOD B, by means of two different photosensitizers (PS) from different chemical classes with different 1O2 quantum yields: methylene blue (MB) and 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP). Mutants, which lack antioxidant enzymes, were particularly susceptible towards TI-PACT. In the case of PACT with MB, quenching agents such as superoxide dismutase (SOD) and catalase (CAT) were sufficient for protecting both the wild type and the mutant, whereas they were not in PAC...Continue Reading

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Citations

Nov 6, 2020·Photochemistry and Photobiology·Fatemeh FayyazRahmatollah Rahimi
Jun 1, 2021·Frontiers in Medicine·Aleksandra Rapacka-ZdończykJoanna Nakonieczna

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

LightCycler
GraphPad Prism

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