PMID: 15434Jan 1, 1977

Susceptibility of various cell lines to virulent an attenuated strains of pseudorabies virus at supraoptimal temperature

Acta Virologica
F GolaisJ Svobodová

Abstract

Replication of virulent and attenuated strains of pseudorabies virus (PRV) at supraoptimal temperature was studied in rabbit lung (ZP), pig kidney (PS), and BHK-21 cells adapted and non-adapted to 40 degrees C and L, HeLa and human amniotic (Am) cells non-adapted to 40 degrees C. The temperature of 40 degreeC did not influence the type cytopathic effect (CPE) in either adapted or non-adapted cells. According to the susceptibility at 40 degrees C the cells could be divided into three groups: cells permissive for all PRV strains (adapted and non-adapted ZP and non-adapted BHK-21 and PS); cells non-permissive for all PRV strains (L, HeLa and Am); and cells permissive for virulent and non-permissive for attenuated PRV strains (adapted BHK-21 and PS). The virus titres in permissive cells at 40 degrees C did not differ from those obtained at 37 degrees C. The attenuated strain CK-PRV X was found to be a tsmutant of PRV with host range character.

Related Concepts

Cytopathogenic Effect, Viral
Thymic Group Viruses
Herpesvirus 1, Suid
Pathogenicity
Virus Replication

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