Susceptibility to virus-cell fusion at the plasma membrane is reduced through expression of HIV gp41 cytoplasmic domains

Virology
Katharina MalinowskyMatthias T Dittmar

Abstract

The cytoplasmic tail of the HIV transmembrane protein plays an important role in viral infection. In this study we analyzed the role of retroviral cytoplasmic tails in modulating the cytoskeleton and interfering with virus-cell fusion. HeLaP4 cells expressing different HIV cytoplasmic tail constructs showed reduced acetylated tubulin levels whereas the cytoplasmic tail of MLV did not alter microtubule stability indicating a unique function for the lentiviral cytoplasmic tail. The effect on tubulin is mediated through the membrane proximal region of the HIV cytoplasmic tail and was independent of membrane localization. Site-directed mutagenesis identified three motifs in the HIV-2 cytoplasmic tail required to effect the reduction in acetylated tubulin. Both the YxxPhi domain and amino acids 21 to 45 of the HIV-2 cytoplasmic tail need to be present to change the level of acetylated tubulin in transfected cells. T-cells stably expressing one HIV-2 cytoplasmic tail derived construct showed also a reduction in acetylated tubulin thus confirming the importance of this effect not only for HeLaP4 and 293T cells. Challenge experiments using transiently transfected HeLaP4 cells and T cells stably expressing an HIV cytoplasmic tail constr...Continue Reading

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Citations

Feb 25, 2011·Molecular Biology of the Cell·Laura García-ExpósitoAgustín Valenzuela-Fernández
Dec 23, 2015·Viruses·Thomas Swaine, Matthias T Dittmar
Jun 25, 2015·Retrovirology·María-Soledad ValeraAgustín Valenzuela-Fernández
Apr 28, 2016·Pharmacology & Therapeutics·Linlin ZhangJun Zhou
Sep 23, 2011·AIDS Research and Human Retroviruses·Tadashi WatanabeJun Komano

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