PMID: 8601725Feb 1, 1996Paper

Suspension-induced murine keratinocyte differentiation is mediated by calcium

The Journal of Investigative Dermatology
L LiS H Yuspa

Abstract

Modulating extracellular Ca2+ (Cao) and suspension culture are two frequently used methods to induce maturation of cultured human and mouse keratinocytes. To determine if the two methods share a common mechanism, changes in Ca2+ metabolism were studied in suspension cultures of mouse keratinocytes. Spontaneously detached and suspension- cultured keratinocytes in 0.05 microM Ca2+ medium express markers of suprabasal differentiation, while 0.05 microM Ca2+ is not permissive for marker expression by attached keratinocytes. Intracellular free Ca2+ (Cai) increased rapidly after placing keratinocytes in suspension in 0.05 microM Ca2+, reaching levels up to 3- to 4-fold higher than Cai in attached cells after 4-5 h. In suspended cells, the increase in Cai was associated with a 2- to 6- fold increase in Ca2+ transport across plasma membrane as well as depletion of intracellular Ca2+ -stores. Differentiation marker expression and terminal differentiation were inhibited in suspension-cultured keratinocytes by preventing the rise of Cai using either 1,2-bis (o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid to chelate intracellular Ca2+ or ethyleneglycol-bis (beta-aminoethyl ether)- N,N,N',N' -tetraacetic acid to reduce Cao. Together thes...Continue Reading

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Citations

Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·M P SantiniG P Dotto
Nov 25, 2003·Clinical and Experimental Immunology·A A KirkaldyD N J Lockwood
Aug 24, 2007·Clinical and Experimental Dermatology·P PaquetG E Piérard
Jun 5, 2008·Journal of Cellular Biochemistry·Orit BossiTamar Tennenbaum
Mar 21, 2019·Journal of Cellular Biochemistry·Jeniffer Farias Dos SantosViviane Abreu Nunes
Mar 12, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniela KovacsGiorgia Cardinali
May 2, 2001·European Journal of Oral Sciences·M MustafaT Modéer
Jan 28, 2003·The Journal of Biological Chemistry·Alisa VespaLina Dagnino
Jul 24, 2008·Archives of Dermatological Research·Justyna Niderla-BielinskaStanislaw Moskalewski
Jan 14, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G P Dotto
Jul 18, 2000·Experimental Cell Research·M K KangN H Park
Jul 8, 2000·The Journal of Investigative Dermatology·M A SleemanJ G Murison
Oct 9, 1998·The Journal of Investigative Dermatology·S SegaertR Bouillon
Apr 14, 1999·The Journal of Investigative Dermatology·M SeishimaY Kitajima
Dec 24, 1997·The Journal of Investigative Dermatology·D PeusM R Pittelkow
Jun 1, 1997·The Journal of Investigative Dermatology·T MauroP A Pappone
Feb 1, 2005·European Journal of Cell Biology·Maranke I Koster, Dennis R Roop

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