Sustainable reduction of bioreactor contamination in an industrial fermentation pilot plant

Journal of Bioscience and Bioengineering
B JunkerP Salmon

Abstract

Facility experience primarily in drug-oriented fermentation equipment (producing small molecules such as secondary metabolites, bioconversions, and enzymes) and, to a lesser extent, in biologics-oriented fermentation equipment (producing large molecules such as recombinant proteins and microbial vaccines) in an industrial fermentation pilot plant over the past 15 years is described. Potential approaches for equipment design and maintenance, operational procedures, validation/verification testing, medium selection, culture purity/sterility analysis, and contamination investigation are presented, and those approaches implemented are identified. Failure data collected for pilot plant operation for nearly 15 years are presented and best practices for documentation and tracking are outlined. This analysis does not exhaustively discuss available design, operational and procedural options; rather it selectively presents what has been determined to be beneficial in an industrial pilot plant setting. Literature references have been incorporated to provide background and context where appropriate.

References

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Citations

May 27, 2011·Current Opinion in Biotechnology·Patrick J Westfall, Timothy S Gardner
Jan 22, 2010·Biotechnology and Bioengineering·Zhihong Fu, Mark T Holtzapple
Mar 20, 2015·Biotechnology and Bioengineering·Thomas O Worley-MorseClaudia K Gunsch
Mar 14, 2009·FEMS Yeast Research·Paolo RelliniGianluigi Cardinali
Apr 4, 2017·Biotechnology for Biofuels·Sylvia GildemynLargus T Angenent
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker
Aug 28, 2009·Applied Biochemistry and Biotechnology·Zhihong Fu, Mark T Holtzapple

Related Concepts

Decontamination
Equipment Contamination
Fermentation
Industrial Microbiology
Maintenance
Pilot Projects
Fermentors
Prosthesis Failure Analysis

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