Sustained delivery of chondroitinase ABC by poly(propylene carbonate)-chitosan micron fibers promotes axon regeneration and functional recovery after spinal cord hemisection

Brain Research
Shilei NiJiangang Wang

Abstract

We describe the sustained delivery of chondroitinase ABC (ChABC) in the hemisected spinal cord using polypropylene carbonate (PPC) electrospun fibers with chitosan (CS) microspheres as a vehicle. PPC and ChABC-loaded CS microspheres were mixed with acetonitrile, and micron fibers were generated by electrospinning. ChABC release was assessed in vitro with high-performance liquid chromatography (HPLC) and revealed stabilized and prolonged release. Moreover, the released ChABC showed sustained activity. PPC-CS micron fibers with or without ChABC were then implanted into a hemisected thoracic spinal cord. In the following 4 weeks, we examined functional recovery and performed immunohistochemical analyses. We found that sustained delivery of ChABC promoted axon sprouting and functional recovery and reduced glial scarring; PPC-CS micron fibers without ChABC did not show these effects. The present findings suggest that PPC-CS micron fibers containing ChABC are a feasible option for spinal cord injury treatment. Furthermore, the system described here may be useful for local delivery of other therapeutic agents.

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Citations

Mar 17, 2017·Acta Cirúrgica Brasileira·Jéssica Rodrigues OrlandinValéria Maria Lara
May 11, 2017·Molecular Medicine Reports·Hourong SunMengmeng Tang
Aug 10, 2019·Experimental Neurology·E MuirJ Fawcett
Oct 30, 2020·Frontiers in Bioengineering and Biotechnology·Wenrui QuMinfei Wu
Nov 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Andrea RaspaFabrizio Gelain
Dec 4, 2020·Tissue Engineering. Part B, Reviews·Jiao Jiao LiBin Zhao

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