Sustained effects of blue light on Streptococcus mutans in regrown biofilm

Lasers in Medical Science
Julie Cohen-BerneronOsnat Feuerstein

Abstract

In prior studies, exposure of Streptococcus mutans in biofilm to blue light using high fluences of up to 680 J/cm(2) did not interfere with bacterial capability to reform an initial biofilm; however, a delayed antibacterial effect was observed. Our aim was to determine the sustained effecttts of blue light-emitting diode (LED) curing light on the pathogenicity of the newly formed biofilm. S. mutans were grown to form biofilm that was exposed to blue light (wavelengths, 460-480 nm) for 1, 3, and 7 min (equivalent to 37, 112, and 262 J/cm(2), respectively). Then, bacteria were suspended and allowed to regrow into new biofilms. The regrown biofilms were assessed for bacterial quantification by optical density (OD) measurement and quantitative polymerase chain reaction (qPCR), bacterial viability and extracellular polysaccharide production by fluorescent staining using confocal scanning laser microscopy, acid production by bacteria (acidogenicity), and bacterial survival at low pH (aciduricity) using qPCR. Bacterial growth in the regrown biofilms was increased when samples were previously exposed to light; however, under the confocal microscopy, a higher proportion of dead bacteria and a reduction in polysaccharide production were ...Continue Reading

References

Feb 4, 2003·Nature Reviews. Drug Discovery·David Davies
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael Wilson
Dec 30, 2004·Photochemistry and Photobiology·Osnat FeuersteinErvin I Weiss
Jun 29, 2005·The Journal of Antimicrobial Chemotherapy·Iriana Carla Junqueira ZaninJonathan Pratten
Mar 15, 2006·The Journal of Antimicrobial Chemotherapy·Osnat FeuersteinDoron Steinberg
Aug 18, 2012·Advances in Dental Research·O Feuerstein
Sep 18, 2012·Journal of Photochemistry and Photobiology. B, Biology·Daniella Chebath-TaubOsnat Feuerstein

❮ Previous
Next ❯

Citations

May 18, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yang YangYong-Ouk You
Jan 10, 2017·Photomedicine and Laser Surgery·Idan RedenskiDoron Steinberg
Nov 13, 2019·Lasers in Medical Science·Maayan VakninOsnat Feuerstein
Dec 15, 2019·Dentistry Journal·Grace Gomez Felix GomezRichard L Gregory
Jun 4, 2020·Brazilian Journal of Biology = Revista Brasleira De Biologia·I D C GaloW G Dos Santos
Jan 18, 2021·Journal of Photochemistry and Photobiology. B, Biology·Sherif A MohamadPaul R Cooper
Nov 18, 2017·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Yucheng WangTianhong Dai
Sep 17, 2021·BMC Oral Health·Mor Schneider-RaymanMiriam Shalish

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Journal of Photochemistry and Photobiology. B, Biology
Daniella Chebath-TaubOsnat Feuerstein
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
Zisheng TangZheng Liu
© 2022 Meta ULC. All rights reserved