Sustained expression of heme oxygenase-1 alters iron homeostasis in nonerythroid cells.

Free Radical Biology & Medicine
Cheng LiRoland Stocker

Abstract

Heme oxygenases initiate the catabolism of heme, releasing carbon monoxide, iron, and biliverdin. Sustained induction of heme oxygenase-1 (HO-1) in nonerythroid cells plays a key role in many pathological processes, yet the effect of long-term HO-1 expression on cellular iron metabolism in the absence of exogenous heme is poorly understood. Here we report that in a model nonerythroid cell, both transient and stable HO-1 expression increased heme oxygenase activity, but total cellular heme content was decreased only with transient enzyme expression. Sustained HO-1 activity increased the expression of both the mitochondrial iron importer mitoferrin-2 and the rate-limiting enzyme in heme synthesis, aminolevulinate synthase-1, and it augmented the mitochondrial content of heme. Also, the expression of transferrin receptor-1 and the activities of iron-regulatory proteins 1 and 2 decreased, whereas total labile iron and the regulatory activity of the heme-binding transcription factor Bach1 were unaltered. In addition, stable, but not transient, HO-1 expression decreased the activities of aconitase, as well as increasing proteasomal degradation of ferritin. Together, our results reveal a novel and coordinated adaptive response of none...Continue Reading

References

Jun 2, 1995·The Journal of Biological Chemistry·P R GardnerC W White
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K D Poss, S Tonegawa
Mar 24, 2000·Archives of Biochemistry and Biophysics·F PetratU Rauen
Sep 5, 2002·Biochemical and Biophysical Research Communications·Giovanni Li VoltiNader G Abraham
Oct 11, 2002·Free Radical Biology & Medicine·Or Kakhlon, Z Ioav Cabantchik
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shaw-Fang YetMark A Perrella
Oct 29, 2004·Human Molecular Genetics·Oliver StehlingRoland Lill
Dec 18, 2004·Science·Esther G Meyron-HoltzTracey A Rouault
Dec 21, 2004·Free Radical Biology & Medicine·Jana MehlhaseTilman Grune
Jul 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fritz H Bach
Mar 3, 2006·Nature·George C ShawBarry H Paw
Apr 8, 2006·Physiological Reviews·Stefan W RyterAugustine M K Choi
Jul 18, 2006·Biochimica Et Biophysica Acta·Roland LillUlrich Mühlenhoff
Sep 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Juan P LiuzziRobert J Cousins
Nov 15, 2006·Circulation·Roland Stocker, Mark A Perrella
Jan 24, 2007·The Journal of Biological Chemistry·Alex D SheftelPrem Ponka
Jul 13, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Paolo SantambrogioSonia Levi
Aug 8, 2007·Molecular and Cellular Biology·Yukari Zenke-KawasakiKazuhiko Igarashi
Jun 3, 2009·Circulation·Caroline ChengHenricus J Duckers
Jul 25, 2009·Free Radical Biology & Medicine·David I Brown, Kathy K Griendling

❮ Previous
Next ❯

Citations

Sep 20, 2012·The Biochemical Journal·Lilibeth LancetaJohn W Eaton
Jul 25, 2013·American Journal of Physiology. Endocrinology and Metabolism·Keiichi KondoHideki Katagiri
Jul 18, 2017·Pest Management Science·Selim TerhzazShireen A Davies
Oct 17, 2019·Antioxidants·J Catharina DuvigneauAndrey V Kozlov
May 8, 2020·Molecular Medicine Reports·Chunyan WangZhongjun Zhang
Sep 26, 2015·Oncotarget·Min-Young KwonSu Wol Chung
Dec 24, 2020·International Journal of Molecular Sciences·Donald David Haines, Arpad Tosaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.