Sustained insulin secretory response in human islets co-cultured with pancreatic duct-derived epithelial cells within a rotational cell culture system.

Diabetologia
H E MurrayR Downing

Abstract

Loss of the trophic support provided by surrounding non-endocrine pancreatic cell populations underlies the decline in beta cell mass and insulin secretory function observed in human islets following isolation and culture. This study sought to determine whether restoration of regulatory influences mediated by ductal epithelial cells promotes sustained beta cell function in vitro. Human islets were isolated according to existing protocols. Ductal epithelial cells were harvested from the exocrine tissue remaining after islet isolation, expanded in monolayer culture and characterised using fluorescence immunocytochemistry. The two cell types were co-cultured under conventional static culture conditions or within a rotational cell culture system. The effect of co-culture on islet structural integrity, beta cell mass and insulin secretory capacity was observed for 10 days following isolation. Human islets maintained under conventional culture conditions exhibited a characteristic loss in structural integrity and functional viability as indicated by a diminution of glucose responsiveness. By contrast, co-culture of islets with ductal epithelial cells led to preserved islet morphology and sustained beta cell function, most evident in ...Continue Reading

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Citations

May 24, 2012·Biotechnology & Genetic Engineering Reviews·M'Balu A WebbRoger F James
Aug 20, 2010·Cell Transplantation·Jamal DaoudMaryam Tabrizian
Sep 26, 2012·Cell Transplantation·M A WebbA R Dennison
Jul 20, 2019·PloS One·Sandra Marín-CañasEduard Montanya
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Jul 4, 2021·Journal of Tissue Engineering and Regenerative Medicine·Hilary E MurrayRichard Downing
Aug 26, 2021·Diabetes, Obesity & Metabolism·Michelle B PagetRichard Downing

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