PMID: 8592138Feb 1, 1996Paper

Sustained nicotine exposure differentially affects alpha 3 beta 2 and alpha 4 beta 2 neuronal nicotinic receptors expressed in Xenopus oocytes

Journal of Neurochemistry
Y N HsuL Wecker

Abstract

To determine whether prolonged exposure to nicotine differentially affects alpha 3 beta 2 versus alpha 4 beta 2 nicotinic receptors expressed in Xenopus oocytes, oocytes were coinjected with subunit cRNAs, and peak responses to agonist, evoked by 0.7 or 7 microM nicotine for alpha 4 beta 2 and alpha 3 beta 2 receptors, respectively, were determined before and following incubation for up to 48 h with nanomolar concentrations of nicotine. Agonist responses of alpha 4 beta 2 receptors decreased in a concentration-dependent manner with IC50 values in the 10 nM range following incubation for 24 h and in the 1 nM range following incubation for 48 h. In contrast, responses of alpha 3 beta 2 receptors following incubation for 24-48 h with 1,000 nM nicotine decreased by only 50-60%, and total ablation of responses could not be achieved. Attenuation of responses occurred within the first 5 min of nicotine exposure and was a first-order process for both subtypes; half-lives for inactivation were 4.09 and 2.36 min for alpha 4 beta 2 and alpha 3 beta 2 receptors, respectively. Recovery was also first-order for both subtypes; half-lives for recovery were 21 and 7.5 h for alpha 4 beta 2 and alpha 3 beta 2 receptors, respectively. Thus, the re...Continue Reading

Citations

Dec 13, 1997·Molecular Neurobiology·J Lindstrom
Nov 19, 2002·Journal of Neurobiology·Michael W Quick, Robin A J Lester
Jun 12, 2014·The Journal of Pharmacology and Experimental Therapeutics·Sunil SahdeoDonald C Button
May 16, 2002·British Journal of Pharmacology·Francisco SalaSalvador Sala
Jun 8, 2007·Cellular and Molecular Neurobiology·Enrique L M Ochoa, Jose Lasalde-Dominicci
Jul 26, 2002·Molecular Psychiatry·R D ShytleP R Sanberg
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Patrick O J Covernton, Robin A J Lester
Jul 13, 2004·The Journal of Biological Chemistry·Gretchen Y López-HernándezJosé A Lasalde-Dominicci
Feb 7, 2004·The Journal of Biological Chemistry·Jérôme SallettePierre Jean Corringer
Apr 12, 2000·Progress in Neurobiology·D Paterson, A Nordberg

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