Sustained oscillations of insulin, glucagon, and somatostatin from the isolated canine pancreas during exposure to a constant glucose concentration

The Journal of Clinical Investigation
J I StagnerG C Weir

Abstract

Canine pancreata were perfused in vitro to examine whether hormone cycles could be demonstrated without hepatic or central nervous influence. Insulin, glucagon, and somatostatin demonstrated regular sustained cyclic secretion from the in vitro canine pancreas. Oscillations were noted for over 200 min during the infusion of a constant glucose concentration. Insulin demonstrated a 10-min period with a range of 8-12 min/cycle. Somatostatin had a 10-min period with a range of 8-11 min. Glucagon had a period of 8.6 min with range of 6-10 min. These periods do not allow glucagon to be consistently 90 degrees out of phase with insulin and somatostatin. When glucose was increased from 88 to 200 mg/dl, insulin cycles persisted but on an elevated base line, demonstrating that cycles react to glucose changes but are not dependent upon them. Cycles were disrupted by infusions of dopamine, apomorphine, epinephrine, and acetylcholine, but were reestablished. Autonomic blockade by both single and combined infusions of atropine (cholinergic), propranolol, and dibenzyline (adrenergic) had no effect on cycles. These results suggest that, in vitro, there is an intrinsic rhythm of hormone secretion by the pancreas despite a constant glucose level....Continue Reading

Citations

Mar 1, 1981·Diabetologia·E SamolsG C Weir
Jun 1, 1982·Pflügers Archiv : European journal of physiology·J C HenquinW Schmeer
Jan 1, 1984·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·W StapelfeldtE F Pfeiffer
Jan 1, 1992·Life Sciences·J I StagnerR Vogel
Nov 1, 1994·Physiology & Behavior·A E WillingH S Koopmans
Nov 1, 1994·Physiology & Behavior·A E WillingE K Walls
Jan 1, 1991·Pharmacology & Therapeutics·T A Rooney, A P Thomas
Jul 9, 1991·Regulatory Peptides·E C OparaO E Akwari
Apr 1, 1995·Progress in Neurobiology·T Akasu, T Nishimura
May 16, 1984·Biochemical and Biophysical Research Communications·J C HenquinH P Meissner
Mar 14, 2002·Molecular Aspects of Medicine·G A Rutter
May 12, 1988·The New England Journal of Medicine·S O'RahillyD R Matthews
Oct 20, 1988·The New England Journal of Medicine·M Husain
Apr 1, 1997·The British Journal of Nutrition·J WaterhouseD Benton
Nov 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·D K McCulloch, J P Palmer
May 1, 1989·Acta Physiologica Scandinavica·E GrapengiesserB Hellman
Nov 6, 2008·Acta Physiologica·M Rupnik
Apr 1, 1990·The Journal of Clinical Investigation·G C Weir, S Bonner-Weir
Feb 1, 1991·The Journal of Clinical Investigation·J SturisK S Polonsky
Nov 20, 1997·The Journal of Clinical Investigation·J WesterlundP Bergsten
Jun 3, 2011·The Journal of Clinical Investigation·Susumu SeinoKohtaro Minami
Aug 24, 2011·World Journal of Diabetes·Javier EspinoAna B Rodríguez
Sep 17, 2009·Journal of Mathematical Biology·Pasquale Palumbo, Andrea De Gaetano
Sep 10, 2014·Diabetes, Obesity & Metabolism·E Gylfe, A Tengholm
May 19, 2009·Medical Hypotheses·M Reza MirbolookiJonathan R T Lakey
May 12, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Pierre PetitRené Gross
Feb 1, 2013·Annals of the New York Academy of Sciences·G C Weir, S Bonner-Weir
Apr 19, 2005·Biophysical Journal·Morten Gram PedersenArthur Sherman
Jul 1, 1983·Metabolism: Clinical and Experimental·D L Cook
Jul 1, 1992·Diabetes Research and Clinical Practice·K YarimizuR Takaki
May 1, 1987·Diabetic Medicine : a Journal of the British Diabetic Association·P R Bratusch-MarrainW Waldhäusl
Aug 31, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·J C Levy
Aug 31, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·B P Hauffa
Jun 5, 2013·Mathematical Biosciences·Pasquale PalumboAndrea De Gaetano
May 24, 2014·Biophysical Journal·Raghuram DhumpaMichael G Roper
Jul 4, 2006·Biophysical Journal·Craig S NunemakerLeslie S Satin
Sep 1, 2000·Seminars in Cell & Developmental Biology·J T DeeneyB E Corkey
Nov 18, 2000·Journal of Theoretical Biology·I M TolićJ Sturis
Feb 24, 2007·Diabetes·Darren J MichaelRobert H Chow
Nov 12, 2015·PloS One·Andrea De GaetanoSimona Panunzi
Feb 1, 1986·Clinics in Endocrinology and Metabolism·E SamolsG C Weir
Dec 8, 2009·Upsala Journal of Medical Sciences·B Hellman
Oct 6, 2010·Journal of Diabetes Science and Technology·Michael L JohnsonWilliam S Evans
Mar 5, 2004·American Journal of Physiology. Endocrinology and Metabolism·Catherine S MaoEli Ipp
Jan 1, 1991·Annals of the New York Academy of Sciences·D R Matthews
Mar 10, 2005·American Journal of Physiology. Endocrinology and Metabolism·Jaime M GrubertOwen P McGuinness
Dec 15, 2007·Journal of Pineal Research·Elmar Peschke
Dec 31, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·H KangD S Koh
Aug 28, 1999·Endocrinology·J M LinP Bergsten
Mar 1, 2007·The Journal of Clinical Endocrinology and Metabolism·Muhammad Abdul-Ghani, Ralph A DeFronzo
Aug 2, 2007·American Journal of Physiology. Endocrinology and Metabolism·Richard BertramLeslie S Satin
Jun 19, 2019·Physical Biology·Taegeun Song, Junghyo Jo
Sep 2, 2016·Physiological Reviews·David G Nicholls
Feb 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·N PørksenHendrik Vilstrup
Jul 1, 1996·Pflügers Archiv : European journal of physiology·A GomisM Valdeolmillos
Mar 22, 1991·The Journal of Comparative Neurology·A L Kirchgessner, J E Pintar
May 11, 2017·Scientific Reports·Dong-Ho ParkJunghyo Jo
Jun 1, 1997·The American Journal of Physiology·N K YaoT S Kuo
Mar 1, 1981·Diabetologia·E SamolsG C Weir
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·N PørksenO Schmitz
Jun 19, 2001·American Journal of Physiology. Endocrinology and Metabolism·S J VoreP C Butler
Jan 11, 2020·Endocrinology, Diabetes & Metabolism·Marie-Béatrice SaadeSophie Blat
Jun 27, 2000·Diabetes/metabolism Research and Reviews·P Bergsten
Nov 30, 2000·Upsala Journal of Medical Sciences·E GylfeJ Westerlund
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·C B JuhlO Schmitz
May 1, 1985·The American Journal of Physiology·J I Stagner, E Samols
Sep 1, 1981·The American Journal of Physiology·J FoxH Heath
Mar 1, 1994·The American Journal of Physiology·R L DobbinsA D Cherrington
Aug 1, 1994·The American Journal of Physiology·J SturisK S Polonsky
Jan 1, 1981·The American Journal of Physiology·B C HansenG P Schielke
Jul 1, 1985·The American Journal of Physiology·A SirekV Harris
Aug 1, 1986·The American Journal of Physiology·J B JaspanE Van Cauter
Mar 3, 2021·Journal of Mathematical Biology·Alessandro Borri, Andrea De Gaetano
Mar 1, 1988·The American Journal of Physiology·R H SafarikD L Curry
May 1, 1986·The American Journal of Physiology·B D MarshR N Bergman
May 1, 1985·The American Journal of Physiology·J I Stagner, E Samols
Mar 1, 1991·The American Journal of Physiology·C J GoodnerE Samols
Oct 1, 1989·The American Journal of Physiology·R W BergstromC de Haën
Oct 1, 1984·The American Journal of Physiology·D S WeigleC J Goodner
Jan 1, 1982·The American Journal of Physiology·B C HansenS B Pek
Jan 1, 1984·The American Journal of Physiology·P VaitkusV Harris

Related Concepts

Canis familiaris
Glucagon
Glucose, (beta-D)-Isomer
Novolin
Islets of Langerhans
Periodicity
Stilamin

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.