Sustained Release of Immunosuppressant by Nanoparticle-anchoring Hydrogel Scaffold Improved the Survival of Transplanted Stem Cells and Tissue Regeneration

Theranostics
Ruixiang LiJianxin Wang

Abstract

The outcome of scaffold-based stem cell transplantation remains unsatisfied due to the poor survival of transplanted cells. One of the major hurdles associated with the stem cell survival is the immune rejection, which can be effectively reduced by the use of immunosuppressant. However, ideal localized and sustained release of immunosuppressant is difficult to be realized, because it is arduous to hold the drug delivery system within scaffold for a long period of time. In the present study, the sustained release of immunosuppressant for the purpose of improving the survival of stem cells was successfully realized by a nanoparticle-anchoring hydrogel scaffold we developed. Methods: Poly (lactic-co-glycolic acid) (PLGA) nanoparticles were modified with RADA16 (RNPs), a self-assembling peptide, and then anchored to a RADA16 hydrogel (RNPs + Gel). The immobilization of RNPs in hydrogel was measured in vitro and in vivo, including the Brownian motion and cumulative leakage of RNPs and the in vivo retention of injected RNPs with hydrogel. Tacrolimus, as a typical immunosuppressant, was encapsulated in RNPs (T-RNPs) that were anchored to the hydrogel and its release behavior were studied. Endothelial progenitor cells (EPCs), as model ...Continue Reading

Citations

Jan 4, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andrew DunnKoyal Garg
Apr 26, 2019·Journal of Biomaterials Science. Polymer Edition·Rongrong WangZhuoyue Chen
Dec 6, 2020·Nature Reviews. Drug Discovery·Michael J MitchellRobert Langer
Apr 30, 2021·Biotechnology and Bioengineering·Tiam M SaffariAlexander Y Shin

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
Assay
ELISA
density gradient centrifugation

Software Mentioned

Nanosight
PeriScan PIM
Image J
GraphPad
GraphPad Prism
NTA

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