Sustained Release of Minor-Groove-Binding Antibiotic Netropsin from Calcium-Coated Groove-Rich DNA Particles

Pharmaceutics
Hyunsu JeonJong Bum Lee

Abstract

Control of the release properties of drugs has been considered a key factor in the development of drug delivery systems (DDSs). However, drug delivery has limitations including cytotoxicity, low loading efficiency, and burst release. To overcome these challenges, nano or micro-particles have been suggested as carrier systems to deliver chemical drugs. Herein, nano-sized DNA particles (DNAp) were manufactured to deliver netropsin, which is known to bind to DNA minor grooves. The rationally designed particles with exposed rich minor grooves were prepared by DNAp synthesis via rolling circle amplification (RCA). DNAp could load large quantities of netropsin in its minor grooves. An analytical method was also developed for the quantification of netropsin binding to DNAp by UV-visible spectrometry. Moreover, controlled release of netropsin was achieved by forming a layer of Ca2+ on the DNAp (CaDNAp). As a proof of concept, the sustained release of netropsin by CaDNAp highlights the potential of the DNAp-based delivery approach.

References

Sep 27, 1972·Nature: New Biology·A TrouetC De Duve
Sep 20, 2001·Current Pharmaceutical Design·M F BrañaA Ramos
Jan 24, 2003·Nature·Nadrian C Seeman
May 1, 1952·The Journal of General Physiology·A D HERSHEY, M CHASE
Feb 1, 1961·Journal of Molecular Biology·L S LERMAN
Jan 6, 2004·Nature Materials·Yougen LiDan Luo
Jun 14, 2005·Nature Biotechnology·Yougen LiDan Luo
Sep 1, 2005·Journal of the American Chemical Society·Yu HeChengde Mao
Mar 17, 2006·Nature·Paul W K Rothemund
Sep 26, 2006·Nature Materials·Soong Ho UmDan Luo
Aug 19, 2007·Current Medicinal Chemistry·Antonino LauriaAnna Maria Almerico
May 22, 2009·Nature·Shawn M DouglasWilliam M Shih
Oct 8, 2009·Journal of the American Chemical Society·Yonggang KeHao Yan
May 18, 2010·Biomaterials·Lei DongJunfeng Zhang
Dec 21, 2011·Natural Product Reports·Paris L Hamilton, Dev P Arya
Mar 1, 2012·Nature Materials·Jong Bum LeePaula T Hammond
Aug 4, 2012·Journal of Photochemistry and Photobiology. B, Biology·Gul Shahzada KhanDavid Barker
Sep 7, 2012·ACS Nano·Yong-Xing ZhaoBjörn Högberg
Dec 4, 2012·Nature Nanotechnology·Jong Bum LeeDan Luo
Aug 21, 2013·Advances in Protein Chemistry and Structural Biology·Arnab Mukherjee, Wilbee D Sasikala
Jan 5, 2014·Arhiv za higijenu rada i toksikologiju·Katarina MiškovićLjubica Glavaš-Obrovac
Feb 13, 2014·Scientific Reports·Younghyun ChoJinkee Hong
Apr 22, 2015·Journal of Biomolecular Structure & Dynamics·Vasily N DanilevichAndrey L Mulyukin
Jul 24, 2015·Nature·Erik BensonBjörn Högberg
Apr 18, 2016·Nucleic Acids Research·Ali A AlmaqwashiMark C Williams
May 5, 2016·Nanoscale Research Letters·Jaepil JeongJong Bum Lee
May 28, 2016·Science·Rémi VenezianoMark Bathe
Oct 12, 2016·Journal of the American Chemical Society·Pengfei WangChengde Mao
Feb 28, 2017·Chemical Communications : Chem Comm·Yishun HuangLeilei Tian
Dec 16, 2017·Science·Dongran HanPeng Yin
Mar 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ananya PaulW David Wilson

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Citations

Jul 12, 2020·Pharmaceutics·Sybil Obuobi, Nataša Škalko-Basnet
Feb 23, 2021·Advanced Nanobiomed Research·David M Smith, Adrian Keller

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Methods Mentioned

BETA
scanning electron microscopy
transmission
X-ray
Assay

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