PMID: 2108487Mar 1, 1990Paper

Swainsonine, an inhibitor of glycoprotein processing, enhances cytotoxicity of large granular lymphocytes

Scandinavian Journal of Immunology
M Yagita, E Saksela

Abstract

In the present study we investigated the effects of inhibitors of glycoprotein processing on cytotoxicity of human large granular lymphocytes (LGL). The incubation of LGL for 36 h with 0.5 microgram/ml swainsonine (SW), which is an inhibitor of mannosidase II, resulted in the augmentation of cytotoxicity of LGL against an NK-resistant colon carcinoma cell line (Colo-320DM) without increase of binding frequency of LGL to target cells or of cell proliferation. The enhanced cytotoxicity was associated with increased binding of concanavalin A to SW-treated LGL. The augmentation of cytotoxicity was also seen by 1-deoxymannojirimycin (1-DMN), an inhibitor of mannosidase I, but much higher amounts of this agent were needed to get the same level of augmentation as that with SW. Other inhibitors of glycoprotein processing such as castanospermine and 1-deoxynojirimycin (1-DN) did not show any augmentative effects on LGL cytotoxicity. The enhancement of cytotoxicity by SW was abolished by the addition of rabbit anti-human interleukin (IL-2) antibody to the culture. This result suggests that IL-2 is involved in the augmentation of cytotoxicity of LGL by SW. The presence of SW in the culture of LGL together with IL-2 also enhanced LAK gener...Continue Reading

References

Jul 5, 1989·Journal of the National Cancer Institute·S A NewtonK Olden
Aug 1, 1989·Cellular Immunology·M YagitaE Saksela
Oct 1, 1986·The Journal of Experimental Medicine·J R OrtaldoR Overton
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Jan 29, 1988·Biochemical and Biophysical Research Communications·S L WhiteK Olden
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K A WallA D Elbein
Jan 1, 1981·CRC Critical Reviews in Biochemistry·P V Wagh, O P Bahl

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Citations

Jul 16, 2003·Journal of Ethnopharmacology·I M HuezaS L Górniak
Jan 1, 1994·Cancer Investigation·R C Turowski, P L Triozzi
May 12, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ji-Yuan SunQian Yang
Nov 14, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·J-Y SunJ-B Wang
Dec 3, 2014·Toxicology Letters·Jiutao WangShanting Zhao
Oct 25, 2011·Human & Experimental Toxicology·Isis M Hueza, Silvana L Górniak
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rodney MorganBaiyong Li
Apr 20, 2016·Organic & Biomolecular Chemistry·Bao-Chen QianChu-Yi Yu
Feb 2, 2008·The Journal of Organic Chemistry·Jason N AbramsGeorge A O'Doherty

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