Swarming and Swimming Changes Concomitant with Phase Variation in Xenorhabdus nematophilus.

Applied and Environmental Microbiology
A GivaudanNoël Boemare

Abstract

Xenorhabdus spp., entomopathogenic bacteria symbiotically associated with nematodes of the family Steinernematidae, occur spontaneously in two phases. Phase I, the variant naturally isolated from the infective-stage nematode, provides better conditions than the phase II variant for nematode reproduction. This study has shown that Xenorhabdus phase I variants displayed a swarming motility when they were grown on a suitable solid medium (0.6 to 1.2% agar). Whereas most of the phase I variants from different Xenorhabdus spp. were able to undergo cycle of rapid and coordinately population migration over the surface, phase II variants were unable to swarm and even to swim in semisolid agar, particularly in X. nematophilus. Optical and electron microscopic observations showed nonmotile cells with phase II variants of X. nematophilus F1 which lost their flagella. Flagellar filaments from strain F1 phase I variants were purified, and the molecular mass of the flagellar structural subunit was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 36.5 kDa. Flagellin from cellular extracts or culture medium of phase II was undetectable with antiserum against the denatured flagellin by immunoblotting analysis. This s...Continue Reading

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Citations

Jul 10, 2007·Nature Reviews. Microbiology·Erin E Herbert, Heidi Goodrich-Blair
Jun 25, 2010·Molecular Microbiology·Vishal S SomvanshiTodd A Ciche
Sep 1, 2013·Probiotics and Antimicrobial Proteins·Asmat AhmadGires Usup
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Jul 15, 2015·Journal of Bacteriology·Elizabeth A HussaHeidi Goodrich-Blair
Sep 14, 2018·Insects·Maurizio Francesco Brivio, Maristella Mastore
Jan 20, 2006·FEMS Microbiology Ecology·James G Mitchell, Kazuhiro Kogure
Sep 19, 2017·Frontiers in Microbiology·Amaury PayellevilleJulien Brillard
Jul 2, 1998·Applied and Environmental Microbiology·J O ThalerN Boemare
Dec 14, 2005·Applied and Environmental Microbiology·A VolgyiS Forst
Apr 23, 2003·Research in Microbiology·Chikezie I Owuama
Jul 1, 1995·Applied and Environmental Microbiology·N MoureauxN Boemare

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