SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets

Investigative Ophthalmology & Visual Science
Maria VähätupaHannele Uusitalo-Järvinen

Abstract

Oxygen-induced retinopathy (OIR) is the most widely used model for ischemic retinopathies such as retinopathy of prematurity (ROP), proliferative diabetic retinopathy (PDR), and retinal vein occlusion (RVO). The purpose of this study was to perform the most comprehensive characterization of OIR by a recently developed technique, sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics. Control and OIR retina samples collected from various time points were subjected to SWATH-MS and detailed data analysis. Immunohistochemistry from mouse retinas as well as neovascular membranes from human PDR and RVO patients were used for the detection of the localization of the proteins showing altered expression in the retina and to address their relevance to human ischemic retinopathies. We report the most extensive proteomic profiling of OIR to date by quantifying almost 3000 unique proteins and their expression differences between control and OIR retinas. Crystallins were the most prominent proteins induced by hypoxia in the retina, while angiogenesis related proteins such as Filamin A and nonmuscle myosin IIA stand out at the peak of angiogenesis. Majority of the changes in protein expression return to normal at ...Continue Reading

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Citations

Nov 30, 2018·Experimental Dermatology·Tuomo KetomäkiTero A H Järvinen
Feb 13, 2020·Investigative Ophthalmology & Visual Science·Yuhang YangGuoming Zhang
Jul 1, 2020·Frontiers in Pharmacology·Maria VähätupaHannele Uusitalo-Järvinen
Aug 23, 2020·Diagnostics·Arleta WaszczykowskaWojciech Młynarski
May 6, 2021·International Journal of Molecular Sciences·Jingfang BianThomas Chuen Lam
Aug 11, 2021·Investigative Ophthalmology & Visual Science·Yedi ZhouYun Li
Dec 18, 2021·Journal of Proteome Research·Manhong XuJoyce Tombran-Tink

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