Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy

Journal of Chromatography. a
Yang LiuGuido F Pauli

Abstract

TLC-based strategies were proposed in 1979 (Hostettmann et al.) and 2005 (Friesen & Pauli; GUESS method) to minimize the number of partitioning experiments required for countercurrent separation (CCS) solvent system selection. As semi-empirical approaches, both proposed that the K values defining the sweet spot of optimal CCS corresponded to a matching Rf value range from the silica gel TLC plate developed in the organic phase of a biphasic or a corresponding monophasic solvent system. Despite their simplicity, there has been an absence of theoretical support and a deficiency of reported experimental evidence. The present study explores the theory required to develop correlations between Rf and K. All theoretical models surmise that the optimal Rf value range should be centered at 0.5. In order to validate the feasibility of the concept of matching Rf and K values, 43 natural products and six solvent system families were investigated. Out of 62 correlations, 45 resulted in matched Rf and K values. Based on this study, practical guidelines for the TLC-based prediction strategy are provided. These approaches will equip CCS users with an updated understanding of how to apply the TLC-based solvent system selection strategy to accel...Continue Reading

Citations

Jul 12, 2019·The Journal of Antibiotics·Edyta M GrzelakSanghyun Cho
Mar 13, 2021·Journal of Natural Products·Seon Beom KimJames B McAlpine
Mar 9, 2019·Journal of Natural Products·J Brent FriesenGuido F Pauli
May 20, 2020·Journal of Natural Products·Seon Beom KimCharlotte Simmler

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