PMID: 8596720Mar 1, 1996Paper

Swelling of rat mesangial cells induces a Ca2+-dependent Cl- conductance

Pflügers Archiv : European journal of physiology
H PavenstädtR Greger

Abstract

Membrane voltage (Vm) and ion currents of rat mesangial cells in primary culture were measured with the patch-clamp technique in the fast whole-cell configuration. Vm was -44 +/- 1 mV (n = 138). A reduction of the osmolality from 290 to 190 mosmol/kg depolarized Vm from -44 +/- 1 to -29 +/- 1 mV (n = 118) and increased the inward and outward conductances (Gm) from 14 +/- 2 to 39 +/- 4 nS and 13 +/- 2 to 37 +/- 4 nS (n = 84), respectively. During the hypotonicity-induced depolarization the cell capacitance increased significantly from 33 +/- 3 to 42 +/- 4 pF (n = 40). The effect of hypotonic cell swelling on Vm was increased in a bath with a reduced extracellular Cl- of 32 mmol/l (by 71 +/- 4%, n = 23), indicating that a Cl- conductance was activated. The permselectivity of this conductance was I- > or = Br- > Cl-. The Vm response was not affected in the presence of a reduced extracellular Na+ of 5 mmol/l (n = 13) and was inhibited in a solution with reduced extracellular Ca2+ concentration (by 63 +/- 9%, n = 14). In microfluorescence measurements with the Ca2+-sensitive dye fura-2 hypotonic cell swelling induced a sustained increase of the intracellular Ca2+ activity, [Ca2+]i (n = 19). The increase of [Ca2+]i was completely inh...Continue Reading

References

Oct 1, 1992·Physiological Reviews·N A McCarty, R G O'Neil
Mar 1, 1992·Kidney International·M Kashgarian, R B Sterzel
Jan 1, 1990·Annual Review of Physiology·C Montrose-Rafizadeh, W B Guggino
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W H Cliff, R A Frizzell
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Oct 1, 1989·Physiological Reviews·P MenéM J Dunn
Oct 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Schlondorff
Dec 1, 1986·The Journal of Clinical Investigation·T OkudaK Kurokawa
Jan 1, 1984·Annual Review of Physiology·B Sakmann, E Neher
Mar 1, 1983·Kidney International·J I Kreisberg, M J Karnovsky
Mar 1, 1993·Kidney International·W CraeliusC E Palant
Jan 1, 1993·Renal Physiology and Biochemistry·F LangD Häussinger
May 1, 1993·Pflügers Archiv : European journal of physiology·R NitschkeR Greger

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Citations

May 8, 2007·The Journal of Membrane Biology·Biyi ChenWon Kyoo Cho
Mar 18, 2005·Physiology·Rong MaSteven C Sansom

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