SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production.

Nature
Zhiye WangXiuren Zhang

Abstract

Chromatin remodelling factors (CHRs) typically function to alter chromatin structure. CHRs also reside in ribonucleoprotein complexes, but little is known about their RNA-related functions. Here we show that CHR2 (also known as BRM), the ATPase subunit of the large switch/sucrose non-fermentable (SWI/SNF) complex, is a partner of the Microprocessor component Serrate (SE). CHR2 promotes the transcription of primary microRNA precursors (pri-miRNAs) while repressing miRNA accumulation in vivo. Direct interaction with SE is required for post-transcriptional inhibition of miRNA accumulation by CHR2 but not for its transcriptional activity. CHR2 can directly bind to and unwind pri-miRNAs and inhibit their processing, and this inhibition requires the remodelling and helicase activity of CHR2 in vitro and in vivo. Furthermore, the secondary structures of pri-miRNAs differed between wild-type Arabidopsis thaliana and chr2 mutants. We conclude that CHR2 accesses pri-miRNAs through SE and remodels their secondary structures, preventing downstream processing by DCL1 and HYL1. Our study uncovers pri-miRNAs as a substrate of CHR2, and an additional regulatory layer upstream of Microprocessor activity to control miRNA accumulation.

References

Sep 17, 2005·Nature Methods·Anne-Valérie GendrelVincent Colot
Dec 13, 2005·Nature Structural & Molecular Biology·Eric BatschéChristian Muchardt
Aug 3, 2006·Genes & Development·J Michael ThomsonScott M Hammond
Oct 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert S AllenAnthony A Millar
Mar 19, 2008·The Plant Journal : for Cell and Molecular Biology·Smita RamanKlaus Theres
Jun 13, 2008·Nature·Brandi N DavisAkiko Hata
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sascha LaubingerDetlef Weigel
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhicheng DongNina Fedoroff
Apr 29, 2009·Bioinformatics·Kévin DartyYann Ponty
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 25, 2009·Nature·Hiroshi I SuzukiKohei Miyazono
Oct 10, 2009·The EMBO Journal·Nicolás G BolognaJavier F Palatnik
May 4, 2010·Nucleic Acids Research·Zhou DuZhen Su
Mar 25, 2011·Methods in Molecular Biology·Suhua FengMatteo Pellegrini
Jun 21, 2011·Nucleic Acids Research·Satoru MachidaY Adam Yuan
Nov 26, 2011·Algorithms for Molecular Biology : AMB·Ronny LorenzIvo L Hofacker
Jul 25, 2013·The Plant Cell·Kestrel Rogers, Xuemei Chen
Aug 13, 2013·Nature Structural & Molecular Biology·Hongliang ZhuXiuren Zhang
Oct 19, 2013·Nucleic Acids Research·Katarzyna Dorota RaczynskaArtur Jarmolowski
Nov 8, 2013·Nucleic Acids Research·Carsten A RaabeTimofey S Rozhdestvensky
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Dec 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shihao ShenYi Xing
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya KawaguchiTetsuro Hirose
Jun 2, 2015·Cell·Tuan Anh NguyenJae-Sung Woo

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Citations

Mar 16, 2019·Genes·Aleksandra Swida-Barteczka, Zofia Szweykowska-Kulinska
Sep 4, 2019·Nature Communications·Somsakul Pop WongpaleeSteven E Jacobsen
Mar 9, 2019·Annual Review of Plant Biology·Xianwei SongYijun Qi
Apr 27, 2019·The Plant Journal : for Cell and Molecular Biology·Pablo A ManavellaJavier Palatnik
Dec 4, 2019·Nature Plants·Jianbo SongXuemei Chen
Jan 11, 2020·The Plant Journal : for Cell and Molecular Biology·Juan Manuel SacnunNahuel González-Schain
Jan 17, 2020·Genes·Caroline ThoulyGilles Vachon
Jan 24, 2020·Development·Yang YuEric C Lai
May 3, 2019·International Journal of Genomics·Dongdong ZhangKeqiang Wu
Sep 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shengjun LiBin Yu
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Susheel Sagar BhatZofia Szweykowska-Kulinska
Apr 12, 2019·Frontiers in Plant Science·Junli WangGuodong Ren
May 10, 2020·Briefings in Bioinformatics·Haopeng YuShiheng Tao
Jan 26, 2021·Molecular Plant·Di Sun, Xiuren Zhang
Jan 10, 2021·Journal of Integrative Plant Biology·Ze-Ting SongJia-Jia Han
Dec 7, 2018·Methods : a Companion to Methods in Enzymology·Zhiye WangXiuren Zhang
Jan 24, 2021·Journal of Experimental Botany·Jiaying ZhuXiuren Zhang
Mar 30, 2021·Plant Diversity·Minghui MouLigang Chen
Apr 30, 2021·Science Advances·Qi LiXiaoming Zhang
May 12, 2021·Plant Cell Reports·Xianrong ZhouVinay Kumar
May 25, 2021·Computational and Structural Biotechnology Journal·Xiangxiang MengShengjun Li
Jun 6, 2021·Journal of Experimental Botany·Feng XiongSha Li
Nov 28, 2018·Biochemistry·Huong Minh NguyenTuan Anh Nguyen
Dec 21, 2021·Plant Signaling & Behavior·Chenmin HuoRuiju Wang
Dec 14, 2021·Frontiers in Plant Science·Pascual Villalba-BermellGustavo G Gomez

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Datasets Mentioned

BETA
GSE108858

Methods Mentioned

BETA
co-immunoprecipitation
two-hybrid
Y2H
immunoprecipitation
RIP
transgenic
Illumina sequencing
PCR
three-hybrid
Y3H

Software Mentioned

samtools
ImageQuant
Prism
Gel
Cutadapt
Picard
Microprocessor
agriGO
TGIRT
Pro Analyzer

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