Swine centromeric DNA repeats revealed by primed in situ (PRINS) labeling

Cytogenetics and Cell Genetics
C Rogel-GaillardM Vaiman

Abstract

In swine, distinct centromeric satellite DNA families have been described that correspond to either all the metacentric chromosomes except the Y (Mc1) or all the acrocentric chromosomes (Ac2). Using primed in situ (PRINS) labeling, we show here that primers derived from various sequences specifically label the centromeres of different subgroups of chromosomes. Among five primers derived from centromeric sequences of acrocentric chromosomes reported to be very homogeneous, four recognize all the acrocentric chromosomes, whereas one labels prominently chromosome 17. For the metacentric chromosomes, six primers have been derived from several divergent sequences. Among these primers, two recognize all the metacentric chromosomes except 5, 10, and 12. Three other primers label small subsets of metacentric chromosomes, including the X and one or two additional chromosomes. The last primer is specific to chromosome 1. These preliminary results suggest that it should be possible to define specific primers for almost every swine chromosome. Already, some of the primers reported here permit a distinction between swine chromosomes difficult to differentiate without banding, such as the X chromosome and chromosome 9. Therefore, the PRINS t...Continue Reading

Citations

Sep 19, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Martine Yerle-BouissouJoël Gellin
Apr 29, 2008·Micron : the International Research and Review Journal for Microscopy·Filomena AdegaHenrique Guedes-Pinto
Jan 25, 2002·American Journal of Medical Genetics·Philippe CoullinAlain Bernheim
Mar 17, 2007·Annual Review of Phytopathology·Giovanni P MartelliValerian V Dolja
Jul 19, 2020·Animals : an Open Access Journal From MDPI·Barbara Danielak-CzechMarek Babicz

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