Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense

Frontiers in Microbiology
Jing WangHaifeng Ji

Abstract

Weaning stress renders piglets susceptible to pathogen infection, which leads to post-weaning diarrhea, a severe condition characterized by heavy diarrhea and mortality in piglets. Enterotoxigenic Escherichia coli (ETEC) is one of typical strains associated with post-weaning diarrhea. Thus, prevention and inhibition of ETEC infection are of great concern. Probiotics possess anti-pathogenic activity and can counteract ETEC infection; however, their underlying mechanisms and modes of action have not yet been clarified. In the present study, the direct and indirect protective effects of Lactobacillus plantarum ZLP001 against ETEC infection were investigated by different methods. We found that bacterial culture and culture supernatant of L. plantarum ZLP001 prevented ETEC growth by the Oxford cup method, and ETEC growth inhibition was observed in a co-culture assay as well. This effect was suggested to be caused mainly by antimicrobial metabolites produced by L. plantarum ZLP001. In addition, adhesion capacity of L. plantarum ZLP001 to IPEC-J2 cells were observed using microscopy and counting. L. plantarum ZLP001 also exhibited a concentration-dependent ability to inhibit ETEC adhesion to IPEC-J2 cells, which mainly occurred via ex...Continue Reading

References

Oct 28, 1999·FEMS Immunology and Medical Microbiology·E M TuomolaS J Salminen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 6, 2005·Applied and Environmental Microbiology·Domitille Fayol-MessaoudiAlain L Servin
Oct 11, 2005·Biotechnology Letters·Kris De Smet, Roland Contreras
Jul 31, 2007·Molecular Immunology·Edwin J A VeldhuizenHenk P Haagsman
Jan 15, 2008·Clinical and Experimental Immunology·M SchleeK Fellermann
Sep 2, 2008·Developmental and Comparative Immunology·Mick Bailey
Jan 23, 2009·Journal of Gastroenterology·Andrea T BorchersM Eric Gershwin
Feb 17, 2010·Nature Reviews. Microbiology·Sarah LebeerSigrid C J De Keersmaecker
May 2, 2013·Journal of Animal Science and Biotechnology·Joy M CampbellJavier Polo
May 7, 2014·Journal of Visualized Experiments : JoVE·Eileen M DunnePaul Licciardi
Jun 8, 2014·Applied and Environmental Microbiology·A A Guerra-OrdazS M Martín-Orúe
Jan 30, 2015·Critical Reviews in Food Science and Nutrition·L Y M WanH El-Nezami
Jun 17, 2015·FEMS Microbiology Letters·Michèle DelleyWolfram M Brück
Aug 10, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Kathryn E MerrimanCorwin D Nelson
Mar 15, 2016·Frontiers in Microbiology·Alistair D S WalshamStephanie Schüller
Sep 13, 2016·Revista Argentina de microbiología·Ana B BenavidesGabriela N Tenea
Jun 13, 2017·Trends in Microbiology·Raphaële GresseStéphanie Blanquet-Diot
Aug 9, 2017·Bioscience of Microbiota, Food and Health·Jean Daniel Dubreuil

❮ Previous
Next ❯

Citations

Aug 2, 2019·Journal of Food Biochemistry·Chao Tang, Zhaoxin Lu
Apr 1, 2020·International Journal of Molecular Sciences·Alessandra DurazzoAntonello Santini
Jan 18, 2019·Journal of Animal Science and Biotechnology·Robin B GuevarraHyeun Bum Kim
Nov 24, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Shike GengAijun Pan
Jun 15, 2021·Frontiers in Molecular Biosciences·Lili WangWenjuan Wu
Jul 6, 2021·Veterinary World·Chiraprapha TuyarumMonthon Lertworapreecha
Oct 29, 2021·Journal of Applied Microbiology·Abdul RaheemGuangzhi Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
light microscopy

Software Mentioned

Primer Express
GraphPad
SAS
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.