Switch from inhibition to activation of the mitochondrial permeability transition during hematoporphyrin-mediated photooxidative stress. Unmasking pore-regulating external thiols

Biochimica Et Biophysica Acta
V PetronilliFernanda Ricchelli

Abstract

We have studied the mitochondrial permeability transition pore (PTP) under oxidizing conditions with mitochondria-bound hematoporphyrin, which generates reactive oxygen species (mainly singlet oxygen, (1)O(2)) upon UV/visible light-irradiation and promotes the photooxidative modification of vicinal targets. We have characterized the PTP-modulating properties of two major critical sites endowed with different degrees of photosensitivity: (i) the most photovulnerable site comprises critical histidines, whose photomodification by vicinal hematoporphyrin causes a drop in reactivity of matrix-exposed (internal), PTP-regulating cysteines thus stabilizing the pore in a closed conformation; (ii) the most photoresistant site coincides with the binding domains of (external) cysteines sensitive to membrane-impermeant reagents, which are easily unmasked when oxidation of internal cysteines is prevented. Photooxidation of external cysteines promoted by vicinal hematoporphyrin reactivates the PTP after the block caused by histidine photodegradation. Thus, hematoporphyrin-mediated photooxidative stress can either inhibit or activate the mitochondrial permeability transition depending on the site of hematoporphyrin localization and on the natu...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N S KosowerH M Ranney
Apr 15, 1990·Journal of Photochemistry and Photobiology. B, Biology·C Salet, G Moreno
Sep 25, 1998·The Journal of Biological Chemistry·E FontaineP Bernardi
May 30, 2001·Advanced Drug Delivery Reviews·J Morgan, A R Oseroff
Mar 29, 2005·The Journal of Biological Chemistry·Emy BassoPaolo Bernardi
May 10, 2005·The International Journal of Biochemistry & Cell Biology·Fernanda RicchelliValeria Petronilli
May 3, 2006·The FEBS Journal·Paolo BernardiMichael A Forte
Feb 13, 2007·Apoptosis : an International Journal on Programmed Cell Death·Andrea Rasola, Paolo Bernardi
Apr 10, 2007·Nature Cell Biology·Christopher P BainesJeffery D Molkentin
May 19, 2007·Biochimica Et Biophysica Acta·Alessandra ZulianFernanda Ricchelli
Aug 1, 2008·The Journal of Biological Chemistry·Anna W C LeungAndrew P Halestrap

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Citations

Nov 3, 2011·The Journal of Membrane Biology·Xiao-Rong LiuYi Liu
Nov 10, 2010·Journal of Bioenergetics and Biomembranes·Tiffany Greco, Gary Fiskum
Oct 1, 2013·Biological Trace Element Research·Jie ZhaoYi Liu
Oct 1, 2013·Asian Pacific Journal of Tropical Biomedicine·Olusola Olalekan ElekofehintiJoao Batista Teixeira Rocha
Sep 21, 2011·Critical Reviews in Food Science and Nutrition·Francesco VisioliMarvin Edeas
Apr 2, 2016·Oxidative Medicine and Cellular Longevity·Nina Kaludercic, Valentina Giorgio
Oct 20, 2009·Biochimica Et Biophysica Acta·Lucia BiasuttoCristina Paradisi
Aug 12, 2016·The Journal of Membrane Biology·Lu LaiYi Liu
May 3, 2014·International Journal of Molecular Sciences·Manuela AntonielGiovanna Lippe
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Apr 20, 2010·FEBS Letters·Luca AzzolinPaolo Bernardi
Oct 27, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jia WangYi Liu
Jul 9, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sergey M Korotkov

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