Switchable inteins for conditional protein splicing.

Biological Chemistry
Barbara Di Ventura, Henning D Mootz

Abstract

Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses. Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose. Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.

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Citations

Aug 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Vedud PurdeDmitri S Kudryashov
Nov 2, 2019·Nucleic Acids Research·Femi J OlorunnijiSusan J Rosser
Dec 20, 2020·Microorganisms·Ananya NandaSasmita Nayak
Jun 4, 2020·Current Opinion in Biotechnology·Zhi-Gang QianXiao-Xia Xia
Mar 18, 2021·Biochimie·Sunita PandaSasmita Nayak
Nov 28, 2020·Journal of Biochemistry·Giada Forlani, Barbara Di Ventura
Jun 12, 2021·Chemical Society Reviews·Yu-Hsuan TsaiShigeki Kiyonaka
Jun 15, 2021·ACS Omega·Navaneethan PalanisamyBarbara Di Ventura
Jul 29, 2021·Chemical Society Reviews·Alicia E Mangubat-Medina, Zachary T Ball
Aug 20, 2021·Journal of Molecular Biology·Christina Z ChungDieter Söll

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