Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology

Frontiers in Microbiology
Karolina IzbiańskaMagdalena Arasimowicz-Jelonek

Abstract

The study demonstrates protein tyrosine nitration as a functional post-translational modification (PTM) in biology and pathobiology of the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato (Solanum tuberosum L.). Using two P. infestans isolates differing in their virulence toward potato cv. Sarpo Mira we found that the pathogen generates reactive nitrogen species (RNS) in hyphae and mature sporangia growing under in vitro and in planta conditions. However, acceleration of peroxynitrite formation and elevation of the nitrated protein pool within pathogen structures were observed mainly during the avr P. infestans MP 946-potato interaction. Importantly, the nitroproteome profiles varied for the pathogen virulence pattern and comparative analysis revealed that vr MP 977 P. infestans represented a much more diverse quality spectrum of nitrated proteins. Abundance profiles of nitrated proteins that were up- or downregulated were substantially different also between the analyzed growth phases. Briefly, in planta growth of avr and vr P. infestans was accompanied by exclusive nitration of proteins involved in energy metabolism, signal transduction and pathogenesis. Importantly, the P. infestans-potato...Continue Reading

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Citations

Feb 3, 2021·Proceedings. Biological Sciences·Steve KettMassimiliano Marvasi
Nov 12, 2020·International Journal of Molecular Sciences·Joanna GajewskaMagdalena Arasimowicz-Jelonek

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Methods Mentioned

BETA
Assay
ELISA
Electrophoresis
acetylation

Software Mentioned

MASCOT
LSM Image Browser
MultiGauge
Mascot Daemon
Mascot Distiller
Fuji

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