Switching from cut-and-paste to replicative Tn7 transposition

Science
E W May, Nancy L Craig

Abstract

The bacterial transposon Tn7 usually moves through a cut-and-paste mechanism whereby the transposon is excised from a donor site and joined to a target site to form a simple insertion. The transposon was converted to a replicative element that generated plasmid fusions in vitro and cointegrate products in vivo. This switch was a consequence of the separation of 5'- and 3'-end processing reactions of Tn7 transposition as demonstrated by the consequences of a single amino acid alteration in an element-encoded protein essential for normal cut-and-paste transposition. The mutation specifically blocked cleavage of the 5' strand at each transposon end without disturbing the breakage and joining on the 3' strand, producing a fusion (the Shapiro Intermediate) that resulted in replicative transposition. The ability of Tn7 recombination products to serve as substrates for both the limited gap repair required to complete cut-and-paste transposition and the extensive DNA replication involved in cointegrate formation suggests a remarkable plasticity in Tn7's recruitment of host repair and replication functions.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A Shapiro
Oct 1, 1979·Molecular & General Genetics : MGG·A Arthur, D Sherratt
Nov 1, 1991·Molecular Microbiology·N L Craig
May 1, 1990·Journal of Bacteriology·K M Kubo, N L Craig
Feb 1, 1988·Genes & Development·C S Waddell, N L Craig
Nov 7, 1986·Cell·K M Derbyshire, N D Grindley
Dec 1, 1986·Molecular & General Genetics : MGG·M RogersD Sherratt
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·E OhtsuboY Machida
Nov 18, 1983·Science·T A WeinertN D Grindley
Aug 18, 1995·The Journal of Biological Chemistry·H Nakai, R Kruklitis
Mar 11, 1993·Nucleic Acids Research·D L Pérez-Morga, P T Englund

❮ Previous
Next ❯

Citations

Jun 23, 2004·Research in Microbiology·Zita Nagy, Michael Chandler
Apr 3, 2013·Trends in Biotechnology·Coral González-PrietoMatxalen Llosa
Nov 6, 2002·Trends in Plant Science·Damon Lisch
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E RaoN L Craig
May 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ki Young ChoiNancy L Craig
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·A R LoheD L Hartl
Jan 10, 1998·The EMBO Journal·A E Stellwagen, N L Craig
Jun 2, 2001·The EMBO Journal·N P Tavakoli, K M Derbyshire
Feb 17, 2001·The EMBO Journal·P N KuduvalliN L Craig
Apr 15, 2008·Applied and Environmental Microbiology·Christophe BordiJohn D Helmann
Jan 1, 1997·Annual Review of Biochemistry·N L Craig
Dec 9, 2000·Annual Review of Entomology·P W AtkinsonD A O'Brochta
Aug 29, 2009·PLoS Genetics·Asad Ahmed
Oct 28, 2008·Plasmid·Adam R Parks, Joseph E Peters
Aug 6, 2014·Molecular Microbiology·Joseph E PetersMichael T Petassi
Jun 12, 2010·Journal of Molecular Biology·Jason W Holder, Nancy L Craig
Oct 20, 1998·Cell·A K KennedyD B Haniford
Nov 5, 1999·Annual Review of Microbiology·L HarenM Chandler
Oct 3, 2001·Journal of Medical Entomology·M L AllenC S Levesque
Jan 12, 2001·Journal of Molecular Biology·A E Stellwagen, N L Craig
May 18, 2016·Chemical Reviews·Alison B Hickman, Fred Dyda
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·M Joan Curcio, Keith M Derbyshire
Jul 10, 2004·Immunological Reviews·Jessica M Jones, Martin Gellert
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·J E Peters, N L Craig
Jun 13, 2019·Nature·Sanne E KlompeSamuel H Sternberg
Mar 30, 1999·Molecular Microbiology·N P Tavakoli, K M Derbyshire

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.