Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles.

Bioconjugate Chemistry
Edward J SayersC Alexander

Abstract

Ligand-mediated targeting and internalization of plasma membrane receptors is central to cellular function. These types of receptors have accordingly been investigated as targets to facilitate entry of diagnostic and therapeutic constructs into cells. However, there remains a need to characterize how receptor targeting agents on nanoparticles interact at surface receptors and whether it is possible to control these interactions via exogenous stimuli. Here, we describe the switchable display of the iron-transporting protein, transferrin (Tf), at the surface of thermoresponsive polymer-coated gold nanoparticles and show that internalization of the coated nanoparticles into target cells changes across temperature ranges over which transferrin is expected to be sterically "hidden" by an extended polymer chain and then "revealed" by polymer chain collapse. The switching process is dependent on the numbers of transferrin molecules and thermoresponsive polymer chains attached and whether the assay temperature is above or below the transition temperatures of the responsive polymers at the nanoparticle surfaces. Significantly, however, the control of internalization is critically reliant on overall nanoparticle colloidal stability while...Continue Reading

References

Jul 10, 2008·Molecular Pharmaceutics·Shyh-Dar Li, Leaf Huang
Jul 25, 2008·Journal of the American Chemical Society·Johannes Pall MagnussonCameron Alexander
Jun 16, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Bradley DuncanVincent M Rotello
Oct 23, 2010·Nature Chemistry·Chaekyu KimVincent M Rotello
Nov 19, 2010·Microbial Cell Factories·Christopher J A FinnisDarrell Sleep
May 5, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ju Yen FuChristine Dufès
Aug 3, 2011·Chemical Communications : Chem Comm·Francesca MastrottoStefano Salmaso
Oct 20, 2011·The British Journal of Radiology·S JainJ M O'Sullivan
Dec 7, 2011·Molecular Pharmaceutics·Mahmoud SolimanCameron Alexander
Feb 9, 2012·Chemical Society Reviews·Rubul MoutVincent M Rotello
Apr 21, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eugene MahonKenneth A Dawson
Jun 12, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tian YuHamidreza Ghandehari
Dec 6, 2012·Nature Nanotechnology·Marco P MonopoliKenneth A Dawson
Jan 11, 2013·Journal of the American Chemical Society·Anna LesniakChristoffer Åberg
Jun 17, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sukrut SomaniChristine Dufès
Mar 31, 2015·Nature Nanotechnology·Philip M KellyKenneth A Dawson
Apr 22, 2015·Bioconjugate Chemistry·Chang Soo KimVincent M Rotello
May 23, 2015·Biotechnology and Bioengineering·Nick Frazier, Hamidreza Ghandehari
Sep 29, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Paul R MoodyArwyn T Jones
Aug 31, 2016·ACS Nano·Warren C W ChanWolfgang J Parak
Oct 7, 2017·Bioconjugate Chemistry·Nadezhda A ByzovaBoris B Dzantiev

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