SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections

Journal of Medical Virology
Alessandra Cristina Gomes-RuizBenedito Antônio Lopes da Fonseca

Abstract

Dengue is the most important arthropod-borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real-time polymerase chain reaction may improve diagnosis. TaqMan and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV-3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV-3 was detected and quantitated in 17 IgM-positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV-3, and that for diagnostic purposes, there is no difference between these two assays.

References

Oct 1, 1994·Journal of Virological Methods·S Y ChanS K Lam
Nov 1, 1996·Clinical and Diagnostic Laboratory Immunology·M G Guzmán, G Kourí
Jul 17, 1998·Clinical Microbiology Reviews·D J Gubler
Sep 30, 1998·Lancet·J G Rigau-PérezA V Vorndam
Oct 31, 2001·Memórias do Instituto Oswaldo Cruz·R M NogueiraH G Schatzmayr
Feb 22, 2002·Journal of Medical Virology·David WarrilowGreg A Smith
Jun 29, 2002·BMJ : British Medical Journal·Robert V Gibbons, David W Vaughn
Aug 15, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sérgio Oliveira De PaulaBenedito Antônio Lopes Fonseca
Oct 29, 2002·Lancet·Scott B Halstead, Jacqueline Deen
Dec 7, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Hubert G M Niesters
Aug 22, 2003·Intervirology·Sérgio Oliveira De PaulaBenedito Antônio Lopes da Fonseca
May 28, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sérgio Oliveira De PaulaBenedito Antônio Lopes da Fonseca

❮ Previous
Next ❯

Citations

Jun 26, 2010·Journal of Medical Virology·Luiza A Castro-JorgeBenedito A L Fonseca
Apr 30, 2014·Microbiology and Immunology·Kleber Juvenal Silva FariasBenedito Antônio Lopes da Fonseca
Feb 23, 2013·TheScientificWorldJournal·Kleber Juvenal Silva FariasBenedito Antônio Lopes da Fonseca
Nov 14, 2006·Journal of Neuroscience Methods·Heather S WaltonPeter R Dodd
Oct 18, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Horllys Gomes BarretoVagner Augusto Benedito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.