SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels

Applied Microbiology and Biotechnology
Elodie Barbau-PiednoirNadine Botteldoorn

Abstract

In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well as the STM0296 locus, putatively coding for a cytoplasmic protein. This study includes the design of primer pairs, in silico and in situ selectivity, sensitivity, repeatability and reproducibility evaluations of the seven SYBR®Green qPCR assays. Each detection level displayed a selectivity of 100 %. This combinatory SYBR®Green qPCR system was also compared with three commercially available Salmonella qPCR detection kits. This comparison highlighted the importance of using a multi-gene detection system to be able to detect every target strain, even those with deletion or mutation of important genes.

References

Dec 3, 2014·International Journal of Food Microbiology·Elodie Barbau-PiednoirNancy H Roosens
Jan 23, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F TangZ-Y Sun
Jul 25, 2015·Applied Microbiology and Biotechnology·Véronique WuytsSigrid C J De Keersmaecker
Jun 15, 2014·Journal of Microbiological Methods·Elodie Barbau-PiednoirNadine Botteldoorn
Jan 6, 2015·Applied Microbiology and Biotechnology·Ru ChenYong-Chang Cao
Feb 21, 2018·Applied Microbiology and Biotechnology·Elodie Barbau-PiednoirNancy H Roosens
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Aug 10, 2018·World Journal of Gastroenterology : WJG·Jing-Bo WangHai-Tao Wang
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Related Concepts

Real-Time Polymerase Chain Reaction
Bacterial Proteins
Organic Chemicals
Kinetic Polymerase Chain Reaction
Gene Deletion Abnormality
Bacteriological Techniques
Salmonella
Oligonucleotide Primers
Deletion Mutation
SYBR Green I

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