Sycamore amyloplasts can import and process precursors of nuclear encoded chloroplast proteins

Biochemical and Biophysical Research Communications
K StrzalkaT Akazawa

Abstract

Amyloplasts isolated from white-wild suspension-cultured cells of sycamore (Acer pseudoplatanus L.) are found to import and process the precursor of the small subunit (pS) of ribulose-1,5-bisphosphate carboxylase/oxygenase of spinach, but they lack the ability to form its holoenzyme due to the absence of both the large subunit and its binding-protein. They also import the precursor of the 33-kDa extrinsic protein (p33-kDa) of the O2-evolving complex of Photosystem II from spinach, but process is only to an intermediate form (i33-kDa). Chloroplasts from green-mutant cells of sycamore process p33-kDa to its mature form in this heterologous system. These results suggest that the thylakoid-associated protease responsible for the second processing step of p33-kDa is missing in amyloplasts, possibly due to the absence of thylakoid-membranes. In contrast, the apparent import of the precursor of the light-harvesting chlorophyll a/b-binding apoprotein (pLHCP) from spinach was not detected. Sycamore amyloplasts may lack the ability to import this particular thylakoid-protein, or rapidly degrade the imported molecules in the absence of thylakoid-membranes for their proper insertion.

References

Feb 1, 1986·Archives of Biochemistry and Biophysics·E MinamiA Watanabe
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Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Watanabe, C A Price
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G W Schmidt, M L Mishkind

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Citations

Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
May 22, 2013·Trends in Plant Science·Hsou-min Li, Yi-Shan Teng
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J NgernprasirtsiriT Akazawa
Aug 1, 1991·European Journal of Biochemistry·J JoyardR Douce
Mar 12, 2008·The FEBS Journal·Allan StensballeKaren G Welinder
Jan 1, 1988·Photosynthesis Research·M L Mishkind, S E Scioli
Jun 27, 2018·Photosynthesis Research·Chiung-Chih Chu, Hsou-Min Li

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