Abstract
Backgrounds. Doxorubicin (DOX) is an effective therapeutic drug for malignant tumors; however, its clinical applications were limited by its side effects, especially the cardiotoxicity caused by ROS-mediated p53 and MAPK signal pathways' activation-induced cell apoptosis. Sanyang Xuedai mixture (SYKT) has been reported as an antioxidant agent and attenuated DOX-induced cardiotoxicity by targeting ROS-mediated apoptosis, but the mechanisms are still not fully delineated. Objective. This study aimed at investigating whether SYKT alleviated DOX-induced cardiotoxicity by inhibiting ROS-mediated apoptosis and elucidating the role of ROS-mediated p53 and MAPK signal pathways' activation in this process. Materials and Methods. Identification, separation, and culture of mouse primary cardiomyocytes. Cells were treated with DOX (1 μM), SYKT (30 mg/mL), or SYKT coupled with DOX. The p53 inhibitor Pifithrin-α (PFT-α), p38/MAPK inhibitor SB203583 (SB), and JNK inhibitor SP600125 (SP) were used as positive control. Western blot was employed to detected p53 and p38 as well as JNK expressions and the activation and translocation of Bax and cytochrome C. Flow cytometer (FCM) was used to detect the mitochondrial membrane potential and cell apop...Continue Reading
References
Aug 14, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Saroj Vadhan-RajRobert S Benjamin
Nov 6, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·J-S BolducC Jumarie
Mar 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·H LouP K Singal
Mar 7, 2009·European Heart Journal·Johan SundströmJohan Arnlöv
Nov 17, 2009·Biochemical and Biophysical Research Communications·U K Misra, S V Pizzo
Mar 10, 2011·Nanomedicine·Giuseppe Visani, Alessandro Isidori
Jan 10, 2012·Journal of Molecular and Cellular Cardiology·Xu WangJoseph Loscalzo
Apr 2, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Yun-Xi PanJi-Xin Shi
Oct 10, 2013·The International Journal of Neuropsychopharmacology·Andres UrrutiaMaria Isabel Colado
Nov 19, 2013·Cancer Research·Dong Hoon ShinJong-Wan Park
Dec 18, 2013·British Journal of Pharmacology·Jun-Xu LiYanan Zhang
Jan 15, 2014·Cell Death and Differentiation·Y ShiG Selivanova
Feb 1, 2014·Oncology Reports·Shilpi SahaGaurisankar Sa
Apr 3, 2014·Apoptosis : an International Journal on Programmed Cell Death·Peng LiTakashi Kondo
Sep 3, 2014·Chemical Reviews·Keith U Ingold, Derek A Pratt
May 20, 2015·International Journal of Molecular Sciences·Zhicheng WangYuan Lu
May 23, 2015·PloS One·Min ZhangMamoru Harada
May 24, 2015·Biochemical and Biophysical Research Communications·Jing LiuDeling Yin
Jul 15, 2015·Carcinogenesis·Sara BeccaficoGuglielmo Sorci
Jul 15, 2015·Experimental Cell Research·Guiqi QinTongsheng Chen
Aug 1, 2015·Cytotherapy·Nara YoonRyang Hwa Lee
Mar 6, 2017·Molecular Medicine Reports·Ting ChenZhi-Jie Liu
Mar 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Li WangZhijie Liu
Apr 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mary Chacko SunithaKottayath Govindan Nevin
Apr 15, 2018·Journal of Molecular and Cellular Cardiology·Cristina CarresiVincenzo Mollace
May 9, 2018·ACS Applied Materials & Interfaces·Xiao WeiShaobing Zhou