PMID: 7025902Apr 6, 1981Paper

Symmetrical kinetic parameters for 3-O-methyl-D-glucose transport in adipocytes in the presence and in the absence of insulin

Biochimica Et Biophysica Acta
L P Taylor, G D Holman

Abstract

3-O-Methyl-D-glucose transport in isolated adipocytes in the presence of insulin is symmetric in zero-trans experiments (transport into sugar-free solutions). K oi zt = 6.10 +/- 1. 65 mM, V oi zt = 1.20 +/- 0.19 mM/s; K io zt = 2.66 +/- 0.26 mM, V io zt = 1.19 +/- 0.07 mM/s. (The superscripts o and i and subscript zt refer to outside, inside and zero-trans conditions, respectively). In the absence of insulin K oi zt = 5.41 +/ 0.98 mM, V oi zt = 0.034 +/- 0.014 mM/s; K io zt = 4.09 +/- 1.05 mM, V io zt = 0.153 +/- 0.023 mM/s. For insulin pre-treated cells, infinite-cis experiments (transport into solutions of varying sugar concentrations) are also symmetric K oi ic = 6.51 +/- 0.83 mM, V oi ic = 0.09 mM/s; K io ic = 3.60 +/- 1.33 mM, V io ic = 1.76 +/- 0.63 mM/S (the subscript ic refers to the infinite-cis condition). In the absence of insulin, K oi ic = 9.03 +/- 3.28 mM, V oi ic = 0.066 +/- mM/s; K io ic = 4.54 +/- 1.32 mM, V io ic = 0.106 +/- 0.026 mM/s. The infinite-cis parameters are shown to be technically easier to measure than the zero-trans parameters. The uses of integrated rate equations for studying rapid transport are demonstrated. The results show that the adipocyte sugar transport system handles 3-O-methyl-D-glucose...Continue Reading

References

Jun 2, 1979·Biochimica Et Biophysica Acta·G D Holman
Feb 2, 1979·Biochimica Et Biophysica Acta·G F Baker, R J Naftalin
Aug 15, 1979·The Biochemical Journal·J D Craik, K R Elliott
Jun 1, 1977·Archives of Biochemistry and Biophysics·R R WhitesellD M Regen
Mar 26, 1976·Molecular and Cellular Biochemistry·M P Czech
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K Suzuki, T Kono
Jan 27, 1964·Biochimica Et Biophysica Acta·D M REGEN, H E MORGAN

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Citations

Dec 1, 1989·Diabetologia·H G Joost, T M Weber
Oct 1, 1993·Baillière's Clinical Endocrinology and Metabolism·W T Garvey, M J Birnbaum
Sep 8, 2000·Bioorganic & Medicinal Chemistry·A TatibouëtG D Holman
Oct 25, 2012·The Journal of Biological Chemistry·Sabrina S Vollers, Anthony Carruthers
Oct 11, 2012·PloS One·Françoise KoumanovGeoffrey D Holman
Nov 22, 2018·The Biochemical Journal·Geoffrey D Holman
Jun 28, 2020·Pflügers Archiv : European journal of physiology·Geoffrey D Holman
Dec 27, 2016·Biophysical Reports·Xuejun C Zhang, Lei Han
May 10, 1996·The Journal of Biological Chemistry·R DauteriveB C Reed
May 15, 2001·American Journal of Physiology. Endocrinology and Metabolism·A E HalsethD H Wasserman
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Jeffry M Leitch, Anthony Carruthers
May 1, 1988·The American Journal of Physiology·N LongoL J Elsas
Mar 5, 1998·The American Journal of Physiology·R M O'DohertyD H Wasserman
Jan 10, 1985·Biochimica Et Biophysica Acta·W D Rees, J Gliemann
May 30, 1984·Biochimica Et Biophysica Acta·J Vinten
Sep 7, 1981·Biochimica Et Biophysica Acta·G D HolmanW D Rees
May 22, 1984·Biochimica Et Biophysica Acta·J GliemannJ A Foley
Nov 17, 1986·Biochimica Et Biophysica Acta·Y Okuno, J Gliemann
Aug 20, 1981·Biochimica Et Biophysica Acta·W D Rees, G D Holman
Feb 8, 1982·Biochimica Et Biophysica Acta·G D Holman, W D Rees
Dec 21, 1981·Biochimica Et Biophysica Acta·G D HolmanW D Rees
Jan 10, 1985·Biochimica Et Biophysica Acta·P J MidgleyG D Holman
Mar 23, 1994·Biochimica Et Biophysica Acta·T J Wheeler
Feb 1, 1987·Molecular and Cellular Endocrinology·J M MayJ M Olefsky
Apr 14, 1982·Biochemical and Biophysical Research Communications·G E LienhardJ C Gorga

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