Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis

Molecular Human Reproduction
Maaike WellingPeriklis Pantazis

Abstract

In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the imaging toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated in vivo with improved spatiotemporal resolution, with more detailed quantitative analyses down to the single-cell level of the developing embryo. Such imaging tools can provide many benefits to investigate the emergence of the asymmetry in the early mammalian embryo. Quantitative single-cell imaging has provided a deeper knowledge of the dynamic processes of how and why apparently indistinguishable cells adopt separate fates that ensure proper lineage allocation and segregation. To advance our understanding of the mechanisms governing such cell fate decisions, we will need to address current limitations of fluorescent probes, while at the same time take on challenges in image processing and analysis. New discoveries and developments in quantitative, single-cell imaging analysis will ultimately enable a truly comprehensive, multi-dimensional and multi-scale investigation of the dynamic morphogenetic processes that work in concert to shape ...Continue Reading

References

Jun 1, 1977·The Journal of Experimental Zoology·S J Kelly
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D AxelrodT R Podleski
Aug 1, 1996·Current Opinion in Genetics & Development·S Grünert, D St Johnston
Sep 25, 1998·Science·M BruchezA P Alivisatos
Aug 17, 1999·Current Opinion in Genetics & Development·F van Eeden, D St Johnston
Sep 14, 2002·Science·George H Patterson, Jennifer Lippincott-Schwartz
Dec 18, 2002·Computers in Biology and Medicine·Tim W NattkemperWalter Schubert
Jun 3, 1961·Nature·A K TARKOWSKI
Sep 18, 2007·Journal of Biomedical Optics·Periklis Pantazis, Marcos González-Gaitán
Nov 1, 2007·Nature Methods·Sally A KimPetra Schwille
Nov 6, 2007·Development·Jens-Erik Dietrich, Takashi Hiiragi
Jul 3, 2008·Cell Stem Cell·Jonathan A NowakElaine Fuchs
Jul 22, 2008·Nature Methods·Khuloud JaqamanGaudenz Danuser
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thouis R JonesDavid M Sabatini
Feb 7, 2009·Journal of Microscopy·M EmmenlauerH Burkhardt
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Myriam HembergerWolf Reik
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samantha A MorrisMagdalena Zernicka-Goetz
Jul 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Periklis PantazisScott E Fraser
Jan 25, 2011·Nature Cell Biology·Nicolas PlachtaPeriklis Pantazis
Apr 20, 2011·Genesis : the Journal of Genetics and Development·Thai V Truong, Willy Supatto
Jul 19, 2011·Nature Methods·Thai V TruongScott E Fraser
Aug 9, 2011·Nature Biotechnology·Jérémie CapouladeMichael Knop
Jan 24, 2012·Cell·Kai Kretzschmar, Fiona M Watt
Mar 7, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·William P DempseyPeriklis Pantazis
May 15, 2012·Cell Cycle·Periklis Pantazis, Tobias Bollenbach
Aug 9, 2012·Development·Adrienne H K RoederElliot M Meyerowitz
Aug 18, 2012·Nature Protocols·Jelena Čulić-ViskotaPeriklis Pantazis
Oct 17, 2012·Nano Letters·Ryuji IgarashiMasahiro Shirakawa
Nov 28, 2012·Current Biology : CB·Inna TabanskyKevin Eggan
Oct 8, 2013·Nature Structural & Molecular Biology·Yusuke MiyanariMaria-Elena Torres-Padilla
Nov 13, 2013·Optics Express·Jing Liu, Joseph Irudayaraj
Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hanhui MaThoru Pederson
Apr 11, 2014·Methods in Molecular Biology·William P DempseyPeriklis Pantazis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Reviews. Molecular Cell Biology
Periklis Pantazis, Willy Supatto
Genesis : the Journal of Genetics and Development
Thai V Truong, Willy Supatto
Methods in Molecular Biology
Sonja Nowotschin, Anna-Katerina Hadjantonakis
© 2021 Meta ULC. All rights reserved