PMID: 8969950Nov 1, 1996Paper

Sympathetic effects on the steroidogenesis and proliferation of adrenocortical cells in vitro

Endocrine Research
C H Wu, M A Holzwarth

Abstract

Recent evidence supports the hypothesis that sympathetic neurons play a significant role in the regulation of adrenocortical cell proliferation and steroidogenesis. Co-cultures of rat adrenocortical and sympathetic ganglion cells have been established to study sympatho-adrenal interactions. In these studies we have compared differentiation, growth and secretion of adrenocortical cells grown in co-culture with those grown alone. Adrenocortical cells were identified using 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) histochemistry or immunocytochemistry; sympathetic neurons were identified using immunocytochemical localization of tyrosine hydroxylase. The sympathetic neurons usually form clusters of 3-10 cells, and extend neurites to adrenocortical cells. Adrenocortical cells continue to proliferate, express 3 beta HSD and sequester lipid droplets. In the co-cultures, the adrenocortical cells are smaller, form larger clusters and show denser 3 beta HSD staining than the adrenocortical cells alone. The presence of the sympathetic neurons enhances adrenocortical cell proliferation as shown by a 2 fold increase in the number of 3 beta HSD(+) cells as well as increased BrdU incorporation after 48 hrs. Steroidogenesis appeared to ...Continue Reading

References

Jan 1, 1990·The Journal of Endocrinology·J P Hinson
Jan 1, 1987·Annals of the New York Academy of Sciences·M A HolzwarthN Kleitman

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