Symptoms of systemic lupus erythematosus are diagnosed in leptin transgenic pigs

PLoS Biology
Junchen ChenXiang Chen

Abstract

Leptin is a well-known adipokine that plays a critical role in immune responses. To further explore the immunological roles of leptin, we developed a transgenic leptin pig controlled by the pig leptin (pleptin) promoter to overexpress leptin. Symptoms typically associated with systemic lupus erythematosus (SLE) were evident in this transgenic pig strain, including anemia, leukopenia, and thrombocytopenia as well as kidney and liver impairment. Histologically, there were increased immunoglobulin G (IgG) levels, elevated antiplatelet antibody (APA) levels, and deposition of immune complexes in the kidney and liver. In addition, anti-double-stranded DNA antibodies (dsDNAs), antinuclear antibodies (ANAs), and antinucleosome antibodies (ANuAs) were all significantly increased in serum immunological examinations. These findings were also accompanied by repression of the regulatory T cell (Treg) ratio. Significantly, glucocorticoid experimental therapies partially relieved the autoimmune responses and bleeding symptoms observed in these transgenic leptin pigs. Together, these results indicate that leptin plays a critical role in the development of autoimmune disorders and demonstrate that our transgenic leptin pigs can act as a valuab...Continue Reading

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Citations

Mar 13, 2020·Immunological Reviews·Xiangyu TengLaurence Morel
Nov 9, 2019·Animal Cells and Systems·Deling JiaoHong-Jiang Wei
Nov 11, 2019·Clinical Rheumatology·Qihang YuanJing Wei
Oct 22, 2020·BioMed Research International·Sha-Sha TaoHai-Feng Pan
Nov 7, 2021·Transgenic Research·Yubo QingHong-Jiang Wei

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Methods Mentioned

BETA
transgenic
ELISA
PCR
flow cytometry
RNA-seq
PCRs
electrophoresis
cesarean section
biopsy
FACS

Software Mentioned

maSigPro
Flowjo
- Quest
Mouse Interventional Scoring System ( MISS
Bowtie
Metacore
Cell
RSEM
GraphPad
Image Pro Plus

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