Synapsin I gene expression in the adult rat brain with comparative analysis of mRNA and protein in the hippocampus

The Journal of Comparative Neurology
R H MelloniL J DeGennaro

Abstract

Synapsin I is the best characterized member of a family of neuron-specific phosphoproteins thought to be involved in the regulation of neurotransmitter release. In this report, we present the first extensive in situ hybridization study detailing the regional and cellular distribution of synapsin I mRNA in the adult rat brain. Both the regional distribution and relative levels of synapsin I mRNA established by in situ hybridization were confirmed by RNA blot analysis. Our data demonstrate the widespread yet regionally variable expression of synapsin I mRNA throughout the adult rat brain. The greatest abundance of synapsin I mRNA was found in the pyramidal neurons of the CA3 and CA4 fields of the hippocampus, and in the mitral and internal granular cell layers of the olfactory bulb. Other areas abundant in synapsin I mRNA were the layer II neurons of the piriform cortex and layer II and V neurons of the entorhinal cortex, the granule cell neurons of the dentate gyrus, the pyramidal neurons of hippocampal fields CA1 and CA2, and the cells of the parasubiculum. In general, the pattern of expression of synapsin I mRNA paralleled those encoding other synaptic terminal-specific proteins, such as synaptophysin, VAMP-2, and SNAP-25, wit...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W B Huttner, P Greengard
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F E BloomP Greengard
Jan 11, 1992·Analytical Biochemistry·R H MelloniL J DeGennaro
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D W Kahn, J M Besterman
Jun 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M BählerP Greengard
Jan 1, 1988·The Journal of Cell Biology·C A Haas, L J DeGennaro
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R LlinásP Greengard
Dec 15, 1986·Biochemical Pharmacology·P De Camilli, P Greengard
Mar 1, 1989·Neurology·J E HamosD A Drachman
Apr 16, 1987·Nature·M Bähler, P Greengard
Sep 1, 1987·The Journal of Cell Biology·T C Petrucci, J S Morrow
Jan 1, 1985·Neurobiology of Aging·S W ScheffS T DeKosky
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Sep 21, 1984·Science·C W Cotman, M Nieto-Sampedro
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E GoelzP Greengard

❮ Previous
Next ❯

Citations

May 26, 1999·The Journal of Comparative Neurology·K RichterE D Gundelfinger
Jul 1, 1995·Neurobiology of Aging·D S HowlandR Siman
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Nicolas G BazanWalter J Lukiw
May 10, 2001·Schizophrenia Research·M J WebsterJ E Kleinman
Aug 13, 1998·Seizure : the Journal of the British Epilepsy Association·K MorimotoT Hayabara
Sep 30, 1998·The European Journal of Neuroscience·M Carrión-VázquezM Nieto-Sampedro
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M MagariñosB S McEwen
Jun 11, 2011·International Journal of Alzheimer's Disease·Pamela Salcedo-TelloClorinda Arias
Jul 6, 2012·The European Journal of Neuroscience·David JeromeQi Yuan
Nov 26, 2009·The Journal of Comparative Neurology·Jianmin SuMichael A Fox
May 30, 1998·Molecular and Cellular Neurosciences·K ArndtC Redies
Feb 26, 2016·Cellular and Molecular Neurobiology·Jingjing Nie, Xiaosu Yang
Dec 29, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R Bernabeu, F R Sharp
May 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R H MelloniR J Harrison
Jul 19, 2008·Stroke; a Journal of Cerebral Circulation·Shang-Wei HouFeng-Yan Sun
Dec 18, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paola FailliDaniele Bani
Sep 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Silvia NistriDaniele Bani
Jul 13, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Avio-Maria PernaDaniele Bani
Jun 19, 1995·The Journal of Comparative Neurology·R H MelloniE L Holzbaur
Jun 10, 1998·Molecular and Cellular Neurosciences·K ArndtC Redies

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.