PMID: 6404912May 1, 1983Paper

Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation

The Journal of Cell Biology
W B HuttnerP De Camilli

Abstract

Synapsin I (protein I) is a neuron-specific phosphoprotein, which is a substrate for cAMP-dependent and Ca/calmodulin-dependent protein kinases. In two accompanying studies (De Camilli, P., R. Cameron, and P. Greengard, and De Camilli, P., S. M. Harris, Jr., W. B. Huttner, and P. Greengard, 1983, J. Cell Biol. 96:1337-1354 and 1355-1373) we have shown, by immunocytochemical techniques at the light microscopic and electron microscopic levels, that synapsin I is present in the majority of, and possibly in all, nerve terminals, where it is primarily associated with synaptic vesicles. In the present study we have prepared a highly purified synaptic vesicle fraction from rat brain by a procedure that involves permeation chromatography on controlled-pore glass as a final purification step. Using immunological methods, synapsin I concentrations were determined in various subcellular fractions obtained in the course of vesicle purification. Synapsin I was found to copurify with synaptic vesicles and to represent approximately 6% of the total protein in the highly purified synaptic vesicle fraction. The copurification of synapsin I with synaptic vesicles was dependent on the use of low ionic strength media throughout the purification. S...Continue Reading

References

Jan 13, 1978·Biochemical and Biophysical Research Communications·R J DeLorenzo, S D Freedman
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Forn, P Greengard
Jun 11, 1976·Brain Research·A NagyV P Whittaker
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W B Huttner, P Greengard
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·U StrömbomP Greengard
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P De CamilliP Greengard
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F E BloomP Greengard
Oct 1, 1978·The Journal of Cell Biology·W S AdairU W Goodenough
Sep 5, 1975·Journal of Molecular Biology·B M Pearse
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E J Nestler, P Greengard
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L K KaczmarekP Greengard
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M B Kennedy, P Greengard
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E GoelzP Greengard
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G FriedP Greengard

❮ Previous
Next ❯

Citations

Jan 1, 1994·Cell Motility and the Cytoskeleton·A JellaliA Rendon
Jan 1, 1994·Cell Motility and the Cytoskeleton·S A KuznetsovG M Langford
Sep 1, 1992·Journal of Neuroscience Research·S CatsicasR J Milner
Apr 24, 2001·Muscle & Nerve·E A Fon, R H Edwards
Oct 23, 1997·Protein Science : a Publication of the Protein Society·C R WangJ Deisenhofer
Feb 1, 1993·Synapse·J L RubensteinA J Czernik
Aug 1, 1997·Cell Biology International·B P JenaK C Sritharan
Sep 1, 1994·Diabetologia·S J Ashcroft
Aug 1, 1986·Journal of Neurocytology·R W BurryW D Matthew
Jun 1, 1989·Journal of Protein Chemistry·S E SeverinE S Severin
Oct 1, 1996·Journal of Neurocytology·G W Balkema, R Rizkalla
Feb 23, 2012·Experimental Brain Research·W Volknandt, M Karas
Sep 2, 2008·Molecular Neurobiology·Zhao-Wen Wang
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·A C AshtonJ O Dolly
Jan 1, 1991·Advances in Enzyme Regulation·S E SeverinV I Kiselev
Aug 20, 1993·Brain Research. Developmental Brain Research·J Y Li, A B Dahlström
Oct 1, 1988·Trends in Neurosciences·S J Smith, G J Augustine
Nov 1, 1989·Trends in Neurosciences·M B Kennedy
May 1, 1992·Brain Research. Molecular Brain Research·J L Bixby
Jan 1, 1993·Brain Research. Molecular Brain Research·R J JohnsonR E Fine
Dec 1, 1992·Neurochemistry International·R J Colbran
Sep 1, 1993·Neurochemistry International·A M CiminiM P Cerú
Jul 1, 1993·Neurochemistry International·F Benfenati, F Valtorta
Nov 1, 1992·Progress in Neurobiology·T Gotow
Mar 1, 1994·Progress in Neurobiology·M VerhageF H Lopes da Silva
Nov 1, 1984·Neuroscience·R F BermanJ E Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.