Synapsins I and II are not required for insulin secretion from mouse pancreatic β-cells

Endocrinology
Anna WendtLena Eliasson

Abstract

Synapsins are a family of phosphoproteins that modulate the release of neurotransmitters from synaptic vesicles. The release of insulin from pancreatic β-cells has also been suggested to be regulated by synapsins. In this study, we have utilized a knock out mouse model with general disruptions of the synapsin I and II genes [synapsin double knockout (DKO)]. Stimulation with 20 mm glucose increased insulin secretion 9-fold in both wild-type (WT) and synapsin DKO islets, whereas secretion in the presence of 70 mm K(+) and 1 mm glucose was significantly enhanced in the synapsin DKO mice compared to WT. Exocytosis in single β-cells was investigated using patch clamp. The exocytotic response, measured by capacitance measurements and elicited by a depolarization protocol designed to visualize exocytosis of vesicles from the readily releasable pool and from the reserve pool, was of the same size in synapsin DKO and WT β-cells. The increase in membrane capacitance corresponding to readily releasable pool was approximately 50fF in both genotypes. We next investigated the voltage-dependent Ca(2+) influx. In both WT and synapsin DKO β-cells the Ca(2+) current peaked at 0 mV and measured peak current (I(p)) and net charge (Q) were of simil...Continue Reading

References

Apr 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·B VonenP Burhol
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K B SeamonJ W Daly
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S ChinP Greengard
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·G JacobssonB Meister
Jun 5, 1998·The Journal of Biological Chemistry·M Hosaka, T C Südhof
Jan 16, 1999·The Journal of Biological Chemistry·K MatsumotoE Miyamoto
Apr 27, 2002·Pflügers Archiv : European journal of physiology·Charlotta S OlofssonLena Eliasson
Feb 27, 2004·Journal of Medical Genetics·C C GarciaJ A Goodship
Dec 17, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel GitlerGeorge J Augustine
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alexander F JeansKay E Davies
Oct 25, 2007·Brain Cell Biology·Melissa VillanuevaR Mark Wightman
Sep 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jenny VikmanLena Eliasson
May 5, 2010·Progress in Neurobiology·F CescaF Benfenati
Jul 28, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brian M KileR Mark Wightman
Jun 7, 2011·Pflügers Archiv : European journal of physiology·Sofia A AnderssonLena Eliasson

❮ Previous
Next ❯

Citations

Aug 24, 2013·Experimental & Molecular Medicine·Michael A Kalwat, Debbie C Thurmond
Dec 8, 2017·Physiological Reviews·Patrik Rorsman, Frances M Ashcroft

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Annals of the New York Academy of Sciences
Matthias BraunPatrik Rorsman
Pflügers Archiv : European journal of physiology
Sofia A AnderssonLena Eliasson
Brain Cell Biology
Melissa VillanuevaR Mark Wightman
Pflügers Archiv : European journal of physiology
K D Gillis, Stanley Misler
© 2022 Meta ULC. All rights reserved