Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse

The Journal of Comparative Neurology
Nuria BesalduchMaria A Lanuza

Abstract

Protein kinase C (PKC) is essential for signal transduction in a variety of cells, including neurons and myocytes, and is involved in both acetylcholine release and muscle fiber contraction. Here, we demonstrate that the increases in synaptic activity by nerve stimulation couple PKC to transmitter release in the rat neuromuscular junction and increase the level of alpha, betaI, and betaII isoforms in the membrane when muscle contraction follows the stimulation. The phosphorylation activity of these classical PKCs also increases. It seems that the muscle has to contract in order to maintain or increase classical PKCs in the membrane. We use immunohistochemistry to show that PKCalpha and PKCbetaI were located in the nerve terminals, whereas PKCalpha and PKCbetaII were located in the postsynaptic and the Schwann cells. Stimulation and contraction do not change these cellular distributions, but our results show that the localization of classical PKC isoforms in the membrane is affected by synaptic activity.

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Citations

Sep 15, 2012·Bulletin of Experimental Biology and Medicine·A E GaydukovO P Balezina
Nov 1, 2012·Bulletin of Experimental Biology and Medicine·A E GaydukovO P Balezina
Feb 24, 2016·Frontiers in Molecular Neuroscience·Suleman HussainSvend Davanger
Jul 11, 2012·The Journal of Comparative Neurology·Adam C LightSteven H DeVries
Feb 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Víctor Cilleros-MañéMaria Angel Lanuza
Feb 1, 2011·The Journal of Comparative Neurology·Núria BesalduchMaria A Lanuza
May 1, 2021·International Journal of Molecular Sciences·Laia Just-BorràsMaria A Lanuza
Jun 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V Cilleros-MañéM A Lanuza

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